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Synergistic stable sodium hyaluronate composite solution composition and preparation thereof

A technology of sodium hyaluronate and composite solution, applied in the field of sodium hyaluronate composite solution composition and its preparation, sodium hyaluronate and its hydrolase inhibitor, can solve the problem of inability to degrade, increase product pushing force, and replenish water Problems such as poor moisturizing performance, to achieve the effect of inhibiting the activity of hyaluronidase, promoting the production of collagen, and prolonging the retention time

Active Publication Date: 2022-07-08
NANJING TZONE BIOLOGICAL SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, although cross-linking modification can delay the degradation and absorption rate of sodium hyaluronate in the body, its moisturizing performance is poor
Moreover, these cross-linked sodium hyaluronate gel products also have relatively large gel strength, and can only be used after being crushed into granules; at the same time, due to the relatively large gel strength and granular appearance of the product itself, the product's The pushing force makes it difficult for the product itself to be injected into the superficial layer through the ultra-fine needle of the hydrolight needle; and the cross-linked sodium hyaluronate gel product has poor fluidity and cannot be evenly distributed on the superficial layer of the hydrolight injection, and it is easy to form bulges and Cannot be degraded and eliminated in a short time
Therefore, there are still many limitations in the current cross-linked sodium hyaluronate gel products for water-light injections

Method used

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  • Synergistic stable sodium hyaluronate composite solution composition and preparation thereof
  • Synergistic stable sodium hyaluronate composite solution composition and preparation thereof
  • Synergistic stable sodium hyaluronate composite solution composition and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] This embodiment is a sodium hyaluronate composite solution composition with synergistic stabilization effect, the composite solution composition comprises non-crosslinked sodium hyaluronate, hyaluronidase inhibitor, solution stabilizer, antioxidant and pH adjusting agent; wherein, the hyaluronidase inhibitor is an amino acid. During our research, we found that under the action of hyaluronidase in the body, sodium hyaluronate is rapidly degraded into small molecular fragments that enter the body fluid circulation for absorption and metabolism, and loses the function of local moisturizing and moisturizing on the skin. Therefore, it is particularly important to find safe excipients that can inhibit the activity of hyaluronidase. Experiments found that amino acids, adenosine heptapeptide, tea polyphenols, and echinacoside can inhibit the activity of hyaluronidase activity, but polyglutamic acid, adenosine heptapeptide, tea polyphenols, echinacoside and Compared with amino ...

Embodiment 2

[0048] This example is to investigate the inhibitory effect of different hyaluronidase inhibitors (ie, different amino acids and concentrations) added to the sodium hyaluronate composite solution composition described in the example on the activity of hyaluronidase and the composition solution. The effect of stability

[0049] The experimental formulas are shown in formulas 1 to 6 in Table 1.

[0050] Table 1. Hyaluronidase inhibitor formulations with different amino acids and concentrations

[0051]

[0052] Preparation process: According to the above formula, each component is weighed and heated to 60°C, stirred and dissolved, and the pH is adjusted to 6.5 with hydrochloric acid. Two complex solution compositions were prepared for each formulation and set aside.

[0053]Hyaluronidase inhibition test: Dissolve bovine testicular hyaluronidase in physiological saline to prepare a solution containing 0.1 mg per 1 ml, add one part of the compound solution composition of the ...

Embodiment 3

[0061] This example includes three experiments, the purpose is to further investigate the inhibitory effect of different amino acid combinations on the activity of hyaluronidase. The experimental formula is shown in Table 3.

[0062] Table 3. Different amino acid formulations

[0063]

[0064] Preparation process: After mixing and preparing each component, it is ready for use.

[0065] In this example, two methods were used to detect the inhibitory effect of different amino acid combinations on the activity of hyaluronidase.

[0066] Experiment 1: Plate Diffusion Detection Enzyme Activity Process: Prepare an agarose plate containing high molecular hyaluronic acid; punch several sample holes with a diameter of 6 mm on the agarose plate with a sterile puncher; Add equal amounts of formulas 7 to 10, as well as blank enzyme solution and water for injection, respectively, with a sample volume of 50 μL. After adding the sample, place the plate horizontally in a 37°C incubator ...

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Abstract

The invention discloses a synergistically stable sodium hyaluronate composite solution composition and a preparation thereof. The sodium hyaluronate composite solution composition comprises non-crosslinked sodium hyaluronate, a hyaluronidase inhibitor, a solution stabilizer, an antioxidant and a pH regulator. Wherein the hyaluronidase inhibitor is amino acid. According to the invention, amino acid is used as a hyaluronidase inhibitor and an auxiliary material for the first time to be combined with non-crosslinked sodium hyaluronate, so that the problems of fast in-vivo hydrolysis and short half-life period of sodium hyaluronate are solved; the purpose of prolonging the in-vivo retention time of the non-crosslinked sodium hyaluronate is achieved, the adopted amino acid serves as a hyaluronidase inhibitor, the good hyaluronidase activity inhibition performance is achieved, safety and reliability are achieved, collagen generation can be effectively promoted while the hyaluronidase activity is inhibited, and the anti-aging effect is achieved. Good medical cosmetology value and economic value are realized.

Description

technical field [0001] The invention belongs to the field of biotechnology, relates to sodium hyaluronate and a hydrolase inhibitor thereof, in particular to a synergistically stable sodium hyaluronate composite solution composition containing a hyaluronidase inhibitor and a preparation thereof. Background technique [0002] Water light acupuncture is a kind of injection-based skin care therapy. When injecting, a special instrument "water light gun" is used to inject the required cosmetic injection into the dermis layer close to the epidermis. After injection, the skin will become hydrated. , named after the light. Water light injection can not only improve skin problems such as wrinkles around the eyes, wrinkles around the mouth, and neck lines caused by dry skin and decreased skin elasticity, but also make the skin increasingly moisturized and rich in moisture. The main purpose of Shuiguang injection is to supplement hyaluronic acid. Hyaluronic acid itself is a natural su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/73A61K8/19A61K8/20A61K8/44A61K8/67A61Q19/00A61Q19/02A61Q19/08
CPCA61K8/735A61K8/447A61K8/44A61K8/675A61K8/673A61K8/676A61K8/678A61K8/19A61K8/20A61Q19/08A61Q19/02A61Q19/00A61K2800/782A61K2800/52A61K2800/522A61K2800/91A61K2800/84
Inventor 徐林金玉徐念沁居丽娜徐松泉曹晶晶
Owner NANJING TZONE BIOLOGICAL SCI & TECH
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