Sterile experiment box for proliferation test of intestinal stem cells of newborn ruminants

Abstract

The invention relates to a sterile experiment box for a new ruminant intestinal stem cell proliferation test. The sterile experiment box comprises a sterile experiment box body, an in-out box is arranged on one side of the sterile experiment box body, when the intestinal tract is placed in a bearing assembly, a lifting baffle, away from one side of the sterile experiment box body, of the in-out box body is firstly opened and closed, then a first in-out opening is closed, and the intestinal tract on the bearing assembly is disinfected and sterilized through a second sterilization lamp in the in-out box body; after the intestinal tract is disinfected and sterilized, a lifting baffle close to one side of the sterile experiment box is opened, the bearing assembly integrally enters the interior of the in-out box, then a second in-out opening is closed, and at the moment, the intestinal tract is observed in the sterile experiment box; one end of the microscope is positioned outside the sterile experiment box, the other end of the microscope is positioned inside the sterile experiment box, and the microscope can be rotatably adjusted, so that the intestinal stem cells inside the sterile experiment box can be conveniently and intuitively observed from the outside.

Description

technical field [0001] The invention belongs to the technical field of aseptic experiment boxes, in particular to a sterile experiment box used for the growth test of intestinal stem cells of newborn ruminants. Background technique [0002] Intestine is an important organ for nutrient digestion, absorption and immune defense of the body. Good intestinal development has a significant impact on ensuring animal health and improving production performance. In recent years, a number of studies using germ-free mice and pigs as model animals for microbiota transplantation have shown that microorganisms are an important factor in promoting intestinal development. Neonatal ruminants are born with a sterile environment in their gut, and gut development is accompanied by microbial colonization and increasing abundance. Therefore, accelerating the establishment of intestinal microflora by microbial transplantation is beneficial to promote intestinal development, but the specific mechan...

AI Technical Summary

Problems solved by technology

Therefore, accelerating the establishment of intestinal microflora through microbial transplantation is beneficial to promote intestinal development, but the specific mechanism of microbial regulation of intestinal development in neonatal ruminants is still unclear

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