Method for predicting feed conversion efficiency of white feather broilers through plasma metabolite abundance modeling
A technology of feed conversion efficiency and feed conversion rate, applied in the field of poultry genetics and breeding, to achieve the effect of saving breeding equipment expenses and reducing breeding costs
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Embodiment 1
[0011] Plasma metabolite markers for identification of feed conversion efficiency in white-feathered broilers
[0012] 1. Experimental materials
[0013] A total of 441 chickens from the 23rd generation of broiler chickens from Northeast Agricultural University with high and low abdominal fat bidirectional selection were used as the experimental population, including 289 low-fat chickens and 152 high-fat chickens.
[0014] 2. Determination of feed conversion ratio and residual feed intake and detection of plasma metabolome
[0015] Record the feed intake FI of each broiler at the age of 29 to 49 days, record the body weight BW4 at the age of four weeks and the body weight BW7 at the age of seven weeks, and calculate the feed conversion ratio FCR and the remaining feed intake RFI. The calculation formula is:
[0016]
[0017]
[0018] where b 0 , b 1 , b 2 is the partial regression coefficient.
[0019] The 48-day-old broilers were cut off from feed and water at 22:0...
Embodiment 2
[0027] Model for predicting feed conversion efficiency in white-feathered broilers by plasma metabolite abundance
[0028] In this example, a model for predicting feed conversion efficiency of white-feathered broilers was established. Taking the 10 biomarkers of feed conversion efficiency identified in Example 1 as independent variables, and the feed conversion ratio and residual feed intake as dependent variables, the data of 431 broilers were randomly selected from the 441 broilers in Example 1 for Modeling. The best regression model was screened out by stepwise regression, and the significant independent variables in the model were retained (P<0.01), and the best model was obtained as follows:
[0029] Predicted value of feed conversion ratio = 2.525 + 4.905 × 10 -5 X 1 +8.970×10 -5 X 2 +1.740×10 -4 X 3 –3.010×10 -4 X 4
[0030] Predicted residual feed intake = –1.679×10 2 +4.848×10 -2 X 1 +1.006×10 -1 X 2 +1.613×10 -1 X 3 –2.655×10 -1 X 4 +1.002×10 -1 X...
Embodiment 3
[0033] Application and effect of prediction model in embodiment 2
[0034]A model for predicting the feed conversion efficiency of white-feathered broilers based on the abundance of plasma metabolites is obtained through Example 2. In this example, the feed conversion efficiency of the remaining 10 chickens in the data of Example 2 is to be identified. The results obtained are as follows: using the feed conversion ratio to identify individuals with high feed conversion efficiency, the ranking is 190>154>251>431>224>441>422>78>43>50, and the real ranking is 190>251>154>224>441>43 >431>422>78>50, the accuracy is 0.85; the ranking of individuals with high feed conversion efficiency using residual feed intake is 381>359>281>353>349>169>216>253>29>27, the real ranking is 381>349>216>359>281>169>353>253>29>27 with an accuracy of 0.75. Therefore, the present invention can better distinguish broilers with high feed conversion efficiency and broilers with low feed conversion efficienc...
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