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Colorimetric detection of target material based on hydrogel particles

A hydrogel particle and colorimetric technology, which is applied in the direction of material inspection products, analytical materials, biological material analysis, etc., can solve problems such as difficult expansion and real-time detection, and achieve high reliability results

Pending Publication Date: 2022-07-15
KOREA UNIV RES & BUSINESS FOUND
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Problems solved by technology

Due to these issues, fluorescence assays should only be performed in centralized laboratories, difficult to scale to point-of-care testing (POCT) where qualitative assays are predominant

Method used

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  • Colorimetric detection of target material based on hydrogel particles
  • Colorimetric detection of target material based on hydrogel particles
  • Colorimetric detection of target material based on hydrogel particles

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experiment example

[0060] 1.1. Fabrication of microfluidic chip

[0061] Microfluidic chips for synthesizing hydrogel particles were designed using AutoCAD (Autodesk, CA, USA) and printed on photomasks (Han & All technology, Korea). SU-8 25 is a negative-tone photoresist, which was coated on the silicon wafer to a thickness of 52 μm, and the SU-8 master pattern was subsequently constructed by photolithography. A mixture of PDMS (Corning, USA) and curing agent in a weight ratio of 10:1 was poured into a SU-8 master mold and set at 70°C for 8 hours. The cured PDMS was separated from the SU-8 master and cut into pieces. Thereafter, the entrance and exit of the channel engraved on the slice were drilled using 1.0 mm and 10.0 mm biopsy punches. A mixture of PDMS and curing agent in a weight ratio of 10:1 was poured onto glass slides and fixed at 70°C for 25 minutes. The prepared sections were attached to the surface of glass slides and cured at 70°C overnight to fabricate microfluidic chips.

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Abstract

The invention relates to the colorimetric detection of a target material based on hydrogel particles. Colorimetric detection of a target material based on hydrogel particles according to an embodiment of the present invention comprises the steps of: reacting a sample comprising the target material with hydrogel particles loaded with a probe specifically binding to the target material; the insoluble colorimetric material is accumulated and amplified in the hydrogel particles to label the target material bound to the probe. Here, the hydrogel particles are made from a polymer network in which the insoluble colorimetric material is immobilized by binding to the polymer network load probe.

Description

technical field [0001] The present invention relates to a method for colorimetric detection of target analytes based on hydrogel particles, and more particularly to a method for detecting target analytes by introducing a colorimetric reaction in hydrogel particles. Background technique [0002] Encoded hydrogel particles have attracted considerable attention in the fields of diagnostic medicine, drug screening, and molecular biochemistry, which require high detection performance for target biomolecules because of their high sensitivity and sensitivity to target biomolecules. Specifically multiplexed. The target analyte bound to the encoded hydrogel particles can be labeled with a fluorescent material. However, the use of expensive systems such as light sources, microscopes, and cameras is critical for fluorescence analysis, making the use of fluorescent materials often difficult where fluorescence analysis systems are not available. Fluorescent materials may lose their flu...

Claims

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Application Information

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IPC IPC(8): G01N33/58G01N33/543G01N33/68
CPCG01N33/581G01N33/5436G01N33/689G01N2800/368
Inventor 奉纪完卢允镐文硕骏李贤智金株延
Owner KOREA UNIV RES & BUSINESS FOUND
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