Supercharge Your Innovation With Domain-Expert AI Agents!

Centrifugal micro-fluidic chip integrating whole blood separation and molecular diagnosis and preparation method of centrifugal micro-fluidic chip

A microfluidic chip and centrifugal technology, applied in the field of microfluidics, can solve problems such as the integration of multiple molecular diagnostics of whole blood samples, and the limitation of the application of centrifugal microfluidic chips.

Pending Publication Date: 2022-07-22
SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
View PDF12 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in the clinical diagnosis of whole blood samples, centrifugal microfluidic chips have been used, but the pretreatment of whole blood samples and the integration of multiple molecular diagnostics have not been realized, which also limits the use of centrifugal microfluidic chips in clinical diagnosis. further application of

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Centrifugal micro-fluidic chip integrating whole blood separation and molecular diagnosis and preparation method of centrifugal micro-fluidic chip
  • Centrifugal micro-fluidic chip integrating whole blood separation and molecular diagnosis and preparation method of centrifugal micro-fluidic chip
  • Centrifugal micro-fluidic chip integrating whole blood separation and molecular diagnosis and preparation method of centrifugal micro-fluidic chip

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0061] The present application also provides a preparation method of the above centrifugal microfluidic chip, the preparation method comprising the following steps:

[0062] The pressure-sensitive adhesive layer is attached to the acrylic sheet, and the pressure-sensitive adhesive layer is cut by laser engraving to form the first siphon valve, the second siphon valve, the third siphon valve, the fourth siphon valve, and the diverter valve. Floor;

[0063] The other pressure-sensitive adhesive layer is attached to another acrylic plate, and the other pressure-sensitive adhesive layer and the other acrylic plate are cut by laser cutting, repeated and stacked to form a chamber including a sample addition chamber, a binding solution chamber, and a washing solution chamber. , other layers of eluent chamber, mixing chamber, detection chamber and waste chamber;

[0064] The first layer and other layers are bonded together to form a centrifugal microfluidic chip.

[0065]Among them,...

Embodiment 1

[0071] This embodiment provides a centrifugal microfluidic chip, refer to figure 1 , the centrifugal microfluidic chip includes a sample adding chamber 110, a binding liquid chamber 120, a washing liquid chamber 130, a second washing liquid chamber 140, an eluent chamber 150, a mixing chamber 160, a predistribution chamber 170, a reaction chamber 180 and a waste The liquid chamber 190 is provided with a corresponding first siphon valve 210 , a second siphon valve 220 , a third siphon valve 230 , a fifth siphon valve 240 , a fourth siphon valve 250 , a ball valve 310 , a diverter valve 320 , and a capillary valve 270 . Among them, the pre-distribution chamber 170 and the reaction chamber 180 are provided with 8 groups.

[0072] In this example, the detection and typing of HBV in blood was also carried out, and the method of RPA-T7-Cas13a was used. The following table shows the sequence and actual dosage of the system.

[0073] Table 1. Sequence of the reaction system

[0074]...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a centrifugal micro-fluidic chip integrating whole blood separation and molecular diagnosis and a preparation method of the centrifugal micro-fluidic chip. On the first aspect, the centrifugal micro-fluidic chip is provided, and the centrifugal micro-fluidic chip comprises a sample adding chamber, a combined liquid chamber, a washing liquid chamber, an eluent chamber, a mixing chamber, a first siphon valve, a second siphon valve, a third siphon valve, a fourth siphon valve, a detection chamber and a waste liquid chamber. According to the centrifugal micro-fluidic chip provided by the embodiment of the invention, different rotating speeds are adjusted through the multi-stage siphon valve, so that a binding solution, a washing solution and an eluent are respectively mixed with a to-be-detected sample, nucleic acid in the sample can be automatically separated, and waste liquid generated in the separation process flows into the waste liquid chamber through the liquid separation valve; and finally separated nucleic acid enters a detection chamber to complete further detection. Through the structural design, the centrifugal micro-fluidic chip can finally complete the whole process of whole blood separation and molecular diagnosis.

Description

technical field [0001] The present application relates to the technical field of microfluidics, in particular to a centrifugal microfluidic chip integrating whole blood separation and molecular diagnosis and a preparation method thereof. Background technique [0002] The current process of separation and detection of free nucleic acid in whole blood mainly includes centrifuge separation, artificial nucleic acid extraction and PCR instrument amplification. This traditional technology not only requires complex instruments and excessive manpower, but also takes a long time. , it will take at least 3 hours to complete the whole process. Taking nucleic acid diagnosis of HBV in clinical whole blood as an example, the whole cycle from sample collection to report can even reach 3-7 days, which seriously affects the clinical diagnosis itself and the subsequent treatment process. [0003] Microfluidic technology has the characteristics of miniaturization, integration, and high throug...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): B01L3/00C12Q1/6844C12Q1/70
CPCB01L3/5027C12Q1/6844C12Q1/70
Inventor 蒋兴宇宗楠
Owner SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com