Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of atp1b1b gene in identification of sea freshwater cultured lateolabrax japonicus and application method

A technology of freshwater aquaculture and application method, applied in the field of identification of atp1b1b gene in sea and freshwater aquaculture, can solve problems such as difficulty in judging the source

Pending Publication Date: 2022-07-22
OCEAN UNIV OF CHINA
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult to judge the source of aquatic products after they are separated from the growth environment, so it is urgent to develop an effective and general method to identify the breeding environment of sea bass

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of atp1b1b gene in identification of sea freshwater cultured lateolabrax japonicus and application method
  • Application of atp1b1b gene in identification of sea freshwater cultured lateolabrax japonicus and application method
  • Application of atp1b1b gene in identification of sea freshwater cultured lateolabrax japonicus and application method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 The ΔCt value of the gill tissue atp1b1b of sea and freshwater cultured sea bass was within the reference range of Table 4.

[0038] (1) Experimental objects:

[0039] A total of 6 seabass with an average weight of 812±25.71g were collected from the sea and freshwater culture of Shuangying Aquatic Seed Co., Ltd. in Lijin County, Dongying City, Shandong Province. After anesthesia treatment with MS-222 (200 mg / L), gill tissue samples were collected and quickly frozen in liquid nitrogen (or placed in RNA protection solution at 4°C overnight), and then stored at -80°C.

[0040] All experimental design and subject handling followed the guidance and approval of the Research and Ethics Committee of Ocean University of China (20141201).

[0041] (2) Experimental method:

[0042] 1. RNA extraction and detection (tissue total RNA extraction reagent-Trizol)

[0043] (1) Take 10-30 mg of perch gonads and add them to a tube containing 1 ml of Trizol. After fully homoge...

Embodiment 2

[0079] Example 2 Gill tissue of sea bass cultured in sea and fresh water atp1b1b Δ Ct values ​​are not within the reference range of Table 4

[0080] (1) Experimental object

[0081] Two market-sized sea bass were collected from sea and freshwater cultured by Shuangying Aquatic Seeds Co., Ltd. in Lijin County, Dongying City, Shandong Province, with an average weight of 857±23.10g. After anesthetized with MS-222 (200mg / L), collect gill tissue samples, wash them with PBS, and fix them with 4% paraformaldehyde (the fixation time depends on the size of the tissue for 6h to 12h overnight, and the time should not be too long), and then dehydrate and soak in wax. Treat or replace with DEPC water to prepare 70% alcohol and store at -20°C.

[0082] All experimental design and subject handling followed the guidance and approval of the Research and Ethics Committee of Ocean University of China (20141201).

[0083] (2) Experimental method

[0084] 1. Probe synthesis

[0085] (1) Prim...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention is applicable to the technical field of biological detection, and provides application and an application method of an atp1b1b gene in identification of sea-freshwater cultured lateolabrax japonicus. As an effective molecular marker for identifying sea freshwater cultured lateolabrax japonicus, the gill tissue expression level of the atp1b1b is closely related to ion transport; by detecting the expression level and the expression position of the atp1b1b gene in the gill tissue of the lateolabrax japonicus, the lateolabrax japonicus individuals cultured in sea and freshwater can be quickly and accurately distinguished.

Description

technical field [0001] The application belongs to the technical field of biological detection, and in particular relates to the application and application method of the atp1b1b gene in the identification of sea bass in freshwater culture. Background technique [0002] Lateolabrax maculatus, belonging to the order Perciformes, Serranidae, and Lateolabrax, commonly known as perch, sea perch, is an important marine economic fish in my country and a typical basal fish. It can adapt to the salinity range of 0 to 45‰. However, studies have shown that freshwater-farmed sea bass are more fishy and less fragrant than seawater. And the content of nutrients such as EPA, DHA and unsaturated fatty acids, the sea bass aquaculture is also significantly higher than the freshwater aquaculture. [0003] Therefore, it is of great significance to distinguish the sea and freshwater aquaculture species of the perch, and to select the perch with higher nutritional content and more delicious tast...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6888C12Q1/6851C12Q1/6841C12N15/11
CPCC12Q1/6888C12Q1/6851C12Q1/6841C12Q2600/166C12Q2531/113C12Q2561/113C12Q2563/107C12Q2545/101
Inventor 李昀温海深齐鑫王灵钰李金库陈通
Owner OCEAN UNIV OF CHINA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com