Use of lone peptide for treating ketamine addiction
A technology of ketamine and orphan, which is applied in the field of biomedicine to achieve the effect of significant drug effect and recovery of physiological functions
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Embodiment 1
[0034] Example 1 Single low dose administration
[0035] A rhesus monkey model of ketamine addiction was established by the following methods:
[0036] The rhesus monkeys were reared alone, provided 3 times a day with adequate monkey feed and 2 times of fruit, free drinking water, and intramuscularly injected ketamine hydrochloride injection to establish a ketamine-addicted rhesus monkey model, including the following steps:
[0037] (a) Dosing period in acute modeling stage: 14 consecutive days, twice a day, each time at a constant dose of 8 mg / kg body weight, intramuscularly inject ketamine hydrochloride injection into rhesus monkeys;
[0038] (b) Transition period of acute modeling stage: on the following day, according to the dose of step (a), intramuscular injection of ketamine hydrochloride injection was given to rhesus monkeys in the morning, and an equal volume of normal saline was intramuscularly injected into rhesus monkeys in the afternoon;
[0039] (c) Forced with...
Embodiment 2
[0069] Example 2 Single high-dose administration
[0070] Three ketamine-addicted rhesus monkey models were randomly divided into 2 groups after modeling, among which: 2 rhesus monkeys in the experimental group were numbered 1 and 2; 1 rhesus monkey in the model group. All rhesus monkeys were housed individually, provided with sufficient monkey feed and 2 times of fruit daily, and had free access to water.
[0071]The experimental group was given orphan at a dose of 5 mg / kg, dissolved with 500 μl of normal saline for injection and then injected intrathecally. The model group was given an equal volume of normal saline for injection.
[0072] All rhesus monkeys were tested for behavior, cortisol (CRF) concentration and orphanin concentration before and after administration of orphan or normal saline. This behavioral test can directly observe the degree of addiction of rhesus monkeys. This plasma cortisol concentration test can evaluate whether orphanin has the potential to res...
Embodiment 3
[0078] Example 3 Continuous low-dose administration
[0079] The three ketamine-addicted rhesus monkey models in Example 2 were constructed as stable ketamine-addiction models again after a 3-month recovery period, and the grouping settings in Example 2 were repeated after modeling. The 3 ketamine-addicted rhesus monkeys were divided into 2 groups, wherein: 2 rhesus monkeys in the experimental group maintained the numbers 1 and 2 in Example 2; 1 rhesus monkey in the model group. All rhesus monkeys were housed individually, provided with sufficient monkey feed and 2 times of fruit daily, and had free access to water.
[0080] The experimental group was given orphan at a dose of 0.5 mg / kg, dissolved with 500 μl of normal saline for injection and then injected intrathecally, while the model group was given an equal volume of normal saline for injection. The dosing interval was 7 days, and the patients were administered 4 times, respectively on the 0th day, the 7th day, the 14th ...
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