Unlock instant, AI-driven research and patent intelligence for your innovation.

Liquid fluorescent dye concentrates and related products and methods for flow cytometry assessment of virus size particles

A technology for flow cytometry, liquid dyes, in the field of liquid fluorescent dye concentrates for flow cytometry assessment of virus size particles and related products and methods

Pending Publication Date: 2022-08-05
SARTORIUS BIOANALYTICAL INSTR INC
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nonetheless, formulating multiple fluorescent dyes in a mixture that has a significant shelf life and can be conveniently used to stain unbound virus-sized particles for flow cytometry assessment with a high level of precision or reproducibility has proven challenging.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Liquid fluorescent dye concentrates and related products and methods for flow cytometry assessment of virus size particles
  • Liquid fluorescent dye concentrates and related products and methods for flow cytometry assessment of virus size particles
  • Liquid fluorescent dye concentrates and related products and methods for flow cytometry assessment of virus size particles

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach

[0076] Some other contemplated embodiments of the various aspects of the present disclosure, with or without the additional features as disclosed above or elsewhere herein, are summarized in the numbered example combinations presented below and in the appended claims:

[0077] 1. A kit for preparing a fluorescently stained fluid sample containing biological material for flow cytometric evaluation of unbound virus-sized particles, said kit comprising:

[0078] A liquid dye concentrate in a sealed first container, the liquid dye concentrate comprising a plurality of different fluorescent dyes in a liquid medium, each of the different fluorescent dyes having different fluorescence emission characteristics for use with all detection when the unbound virus-sized particles are bound; and

[0079] an aqueous sample dilution for preparing an aqueous dilution fluid sample containing biological material for flow cytometric evaluation of unbound virus-sized particles, the aqueous sample ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle sizeaaaaaaaaaa
particle sizeaaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

Kits and methods for flow cytometry comprising a liquid dye concentrate for fluorescently staining virus size particles with a plurality of fluorescent dyes in a liquid medium. The liquid dye concentrate comprises a plurality of fluorescent dyes and one or both of (i) a liquid medium comprising a liquid mixture comprising water and a liquid phase organic material and (ii) a disaccharide dissolved in the liquid medium.

Description

[0001] Related applications [0002] This application is a continuation of US Patent Application No. 16 / 781,782, filed February 4, 2020, entitled "LIQUID FLOURESCENT DYE CONCENTRATEFOR FLOW CYTOMETRY EVALUATION OF VIRUS-SIZE PARTICLES AND RELATED PRODUCTSAND METHODS," which for all purposes is fully The contents are incorporated herein by reference as if fully set forth herein. technical background [0003] Flow cytometry is an analytical technique for measuring the physical and / or chemical properties of particles as they flow in a fluid sample through the study cuvette (often referred to as a flow cell) of a flow cytometer. Although sample fluids can be studied by subjecting them to various stimuli, radiation, usually in the form of light, is a common stimulation technique. Flow cytometry is an important analytical technique that has gained wide acceptance for the analysis of particles in biological materials, especially for studying the properties of cells. More recently, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50G01N1/30G01N15/14
CPCG01N33/50G01N1/30G01N15/1459G01N2015/1438C12Q1/70G01N15/1404G01N33/56983G01N33/582G01N2001/302
Inventor R.K.蒙塔奇J.W.斯蒂芬斯
Owner SARTORIUS BIOANALYTICAL INSTR INC