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Electric sample introduction composite tube for capillary electrophoresis

A technology of capillary electrophoresis and composite tubes, applied in the direction of material analysis, measuring devices, instruments, etc. by electromagnetic means, can solve the problems of unfavorable quantitative analysis, poor repeatability of electric sample injection, etc., to avoid heat convection, improve stability and detection Sensitivity, the effect of eliminating the dead angle of the electric field

Inactive Publication Date: 2006-01-25
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Due to the existence of convection, thermal convection and electric field dead angle caused by air bubbles, the repeatability of conventional electric sampling (including field amplification sampling) is poor, which is not conducive to strict quantitative analysis

Method used

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  • Electric sample introduction composite tube for capillary electrophoresis
  • Electric sample introduction composite tube for capillary electrophoresis
  • Electric sample introduction composite tube for capillary electrophoresis

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Embodiment Construction

[0023] The technical solution of the present invention will be further described below in conjunction with the accompanying drawings.

[0024] The electrokinetic sampling composite tube structure for capillary electrophoresis of the present invention is as follows figure 1 As shown, a double-tube structure with a diaphragm is adopted, mainly including a sample tube 1, an electrode tube 2, a diaphragm 3 and a cap 4. Both the sample tube 1 and the electrode tube 2 are upright structures, and are respectively composed of their respective outer tube walls, tube bottoms 7 , inner tube walls 11 and diaphragm 3 shared by both. The diaphragm 3 arranged between the sample tube 1 and the electrode tube 2 provides a channel for the conduction of liquid between the sample tube 1 and the electrode tube 2 . exist figure 1 In the embodiment of the composite tube with the diaphragm placed below, the diaphragm 3 is arranged between the lower part of the inner tube wall 11 and the bottom 7 of...

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Abstract

This invention relates to an electric sampling multiple tubes for capillary tube electrophoresis, which employs double-tube structure with diaphragm, wherein one is designed for sampling tube, the other is for electrode tube and they are used separately to receive electric sampling capillary tube and platinum electrode. And at the same time a porous diaphragm is located in the common inner tube wall between sampling tube and electrode to avoid the fluid flow between them. The diaphragm porosity is good at conductivity. The bottom of sampling tube is curved connected with its outer wall so as to effectively eliminate electric field blind angle. The invention effectively solves the problem of bubbling on electrode surface and fluid flow caused by heat diversity in traditional electric sampling, including field enlarging sampling.

Description

technical field [0001] The invention relates to an electrodynamic sampling composite tube for capillary electrophoresis, which can be widely used in conventional pressure sampling, gravity sampling and electrodynamic sampling of various samples of capillary electrophoresis, especially for the enrichment of large-volume electrophoresis sampling, improving the Analyze the stability and detection sensitivity of capillary electrophoresis. Background technique [0002] Capillary electrophoresis (CE) is a high-efficiency separation and analysis technique that has been widely used in the separation and analysis of various compounds such as genes, proteins, and drugs. Due to the small inner diameter, the sample volume of conventional CE is generally controlled within 5% of the column volume, and the sample volume is between several to tens of nl. The large-volume injection enrichment technology adopted by Thormann (A.B.Wey, C.-X.Zhang and W.Thormann, J.Chromatogr.A 853:95-106, 1999...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/447G01N27/453G01N30/08
Inventor 曹成喜李杉
Owner SHANGHAI JIAO TONG UNIV
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