Tissue cultivation quick breeding method of grass cherry blossom
A technology of cherry blossoms and tissue culture, which is applied in the field of artificial propagation and cultivation of plants, and can solve the problems of low reproduction rate
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Embodiment 1
[0017] 1. Induction culture of test-tube seedlings: cut the stem tip and stem section of adult grass cherry blossoms, rinse with running water for 1-2 hours; add 0.1% mercury dichloride to disinfect for 3-5 minutes; then rinse with sterile water 3-5 times, Dry the surface with sterile filter paper, cut into 0.3 cm leafy stems or shoot tips, put them on the induction medium: 2368.22 mg / L MS, add 1 mg / L 6-benzylpurine, and place in the culture room After culturing for more than 30 days, the test-tube plantlets grow into test-tube plantlets, and the induction rate of the test-tube plantlets is above 95%.
[0018] 2. Proliferation culture of test-tube seedlings: Transfer the shoot tip or stem segment of about 0.5 cm into the proliferation medium, 2368.22 mg / L MS medium, add 6-benzylpurine 2 mg / L, inositol 100 mg / L , dicloconazole 0.2 mg / L, edible sugar 30 g / L, cornstarch 60 g / L, cultivated for more than 25 days, seedling height 2-4 cm. Repeat the same process, carry out the propa...
Embodiment 2
[0023] Repeat the operation process of Example 1, the test-tube seedling induction medium is: 2368.22 mg / liter of MS medium, 1 mg / liter of 6-furfuryl purine, 0.2 mg / liter of dicloconazole, 30 grams / liter of edible sugar, cornstarch 60 grams per liter; the number of bud proliferation is 6-10, and the bud differentiation rate is above 90%.
[0024] The test-tube seedlings are transferred to the proliferation medium, and the proliferation medium is: 2368.22 mg / L MS medium, 6-benzyl purine 2 mg / L, gibberellin 0.5-1 mg / L, diconazole 0.2 mg / L , edible sugar 30 g / L, cornstarch 60 g / L; cultivated for more than 25 days, the seedling height is 1-4 cm, the number of bud differentiation reaches 8-20, and the differentiation rate is 100%.
[0025] The following operations are the same as Example 1.
Embodiment 3
[0027] Repeat embodiment 1 operation process, in test-tube seedling multiplication process, adopt following medium and result as follows:
[0028] 2368.22 mg / L MS medium, add 1.25 mg / L 6-benzyl purine, the number of bud differentiation is 6-13;
[0029] 2368.22 mg / L MS medium, 6-furfuryl purine 1.5 mg / L, bud differentiation number 6-14;
[0030] During the rooting process, the following media were used and the results were as follows:
[0031] Adding 0.1 mg / L indolebutyric acid and 0.1 mg / L NAA to 1184.11 mg / L MS medium, the rooting rate was 43%;
[0032] 1184.11 mg / L MS medium, adding 0.2 mg / L indole butyric acid, the rooting rate is 58%;
[0033] 1184.11 mg / L MS medium, adding 0.3 mg / L indole butyric acid, the rooting rate is 78%;
[0034] 1184.11 mg / L MS medium, adding 0.2 mg / L indole acetic acid, the rooting rate is 55%;
[0035] 1184.11 mg / L MS medium, adding 0.3 mg / L indole acetic acid, the rooting rate is 73%;
[0036] 1184.11 mg / L MS medium, adding 0.4 mg / L indole...
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