Monoclonal antibody to alpha 2-macroglobulin and its use for detecting glucan

A monoclonal antibody, macroglobulin technology, applied in the field of glucan detection, can solve the problem of not being able to develop analytical glucan and so on

Inactive Publication Date: 2001-01-31
BIOWHITTAKER TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the prior art fails to develop a successful method for the analysis of dextran using amebocyte lysate (LAL)

Method used

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  • Monoclonal antibody to alpha 2-macroglobulin and its use for detecting glucan

Examples

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Embodiment 1

[0041] This example demonstrates the inhibition of the response of amebocyte lysates to endotoxin but not dextran in the presence of monoclonal antibody 7A10.

[0042] The monoclonal antibody 7A10 was added to the Limulus amebocyte lysate to a final concentration of 1.560 μg / ml, 3, 125 μg / ml, and 6.250 μg / ml. The percentage inhibition of the endotoxin response by the lysate was assessed by measuring the inhibition of the cleavage of the chromogenic substrate in the control lysate without mAb and in the lysate samples spiked with different concentrations of mAb. Each lysate sample was challenged by adding 0.5 Eu / ml endotoxin.

[0043] The response of the lysate sample to this level of endotoxin was completely inhibited when the concentration of mAb 7A10 was 6.250 μg / ml ( figure 1 ). Elevated concentrations of a second mAb, 2D5, which also interacts with Limulus α 2 - specifically binds to one epitope on macroglobulin.

[0044] Addition of purified monoclonal antibody 7A10 c...

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Abstract

The present invention is directed to monoclonal antibodies which specifically bind to a Limulus alpha 2-macroglobulin. Preferred monoclonal antibodies inhibit the enzymatic activation of a Limulus amebocyte lysate to form a gel or to cleave a chromogenic substrate in the presence of an endotoxin but not in the presence of a glucan. The monoclonal antibodies can be used, inter alia, to purify a Limulus alpha 2-macroglobulin, and the preferred antibodies can be used in a method for specifically detecting a glucan in a test sample using an amebocyte lysate.

Description

[0001] technical field of invention [0002] The invention relates to the field of dextran detection. More specifically, the present invention relates to the use of dextran from a Limulus amebocyte lysate. Background of the invention [0003] Limulus amebocyte lysate achieves gel block (gel) formation by two enzymatic pathways. These enzymatic pathways comprise a series of serine proteases that are activated in the presence of endotoxin or dextran, and thanks to this activity, amebocyte lysates are widely used assays to detect the presence of endotoxin. For example in water and biological test samples. [0004] However, it is more sensitive than dextran due to the presence of endotoxin. Therefore, the prior art fails to develop a successful method for the analysis of dextran using amebocyte lysate (LAL). When attempting to detect dextran with amebocyte lysates, the presence of endotoxin in even small amounts in the test sample can lead to false positive results. Therefore...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577C07K1/22C07K16/18C07K16/38C07K17/00C12N5/10C12N5/20C12N15/02G01N33/53G01N33/579
CPCC07K16/18Y10S435/975G01N33/5308
Inventor C·J·赫尔布斯特里特M·G·佩佩
Owner BIOWHITTAKER TECH
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