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Application of using chlorine dioxide as disinfecting agent for cultivating glossy ganoderma

A chlorine dioxide and sterilizing agent technology, applied in chemistry, fungi, etc., can solve the problems of high technical requirements and high cost of Ganoderma lucidum cultivation, and achieve the effects of broad sterilization spectrum, simplified production process, and energy saving

Inactive Publication Date: 2007-02-28
SHENZHEN POLYTECHNIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the traditional cultivation of ganoderma lucidum, the prepared medium needs to be sterilized by high temperature, and strict aseptic operation must be adopted in the inoculation and cultivation process. The cost of ganoderma cultivation is high and the technical requirements are high

Method used

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  • Application of using chlorine dioxide as disinfecting agent for cultivating glossy ganoderma
  • Application of using chlorine dioxide as disinfecting agent for cultivating glossy ganoderma
  • Application of using chlorine dioxide as disinfecting agent for cultivating glossy ganoderma

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Embodiment Construction

[0036] The application of chlorine dioxide sterilant in Ganoderma lucidum medium is now illustrated with specific examples.

[0037] Prepare each medium with the following materials:

[0038] 1. Incline medium

[0039] PDA comprehensive medium: potatoes (peeled) 200g, glucose 20g, KH 2 PO 4 0.75g, MgSO 4 0.3g, 20g agar, 1000ml water. And add 7ml of liquid chlorine dioxide to the water.

[0040] 2. Shake Flask Seed Medium

[0041] PDA comprehensive medium: potatoes (peeled) 200g, glucose 20g, KH 2 PO 4 0.75g, MgSO 4 0.3g, water 1000ml. And add 7ml of liquid chlorine dioxide to the water.

[0042] 3. Shake Flask Fermentation Medium

[0043] Soybean flour comprehensive medium: 20g soybean flour, 20g glucose, 2g yeast extract, KH 2 PO 4 0.75g, MgSO 4 0.3g, water 1000ml. And add 7ml of liquid chlorine dioxide to the water.

[0044] 4. Solid cultivation medium

[0045] Bran 96.3%, sucrose 1%, gypsum (CaSO 4 ) 1%, superphosphate 1%, water is added 1000ml per kilogra...

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Abstract

The invention discloses an application of chlorine dioxide as mycelium culture sterilizing agent, which has wide sterilization spectrum and strong sterilizing ability, harmless to human beings and domestic animals and having no effect on the growth of Ganoderma lucidum As.5.65 and 7 kinds of gloosy ganoderma. The culture medium does not need sterilizing, and as soon as the culture medium is added with a proper amount of sterilizing agent, it can be used for glossy ganoderma seed separation, bacterial strain enlarged culturing, and gloosy ganoderma mycelium and amylose fermentation, as well as glossy ganoderma solid planting, largely simplifying the producing procedure of glossy ganoderma culture, saving energy sources, and remarkably reducing production cost.

Description

technical field [0001] The present invention relates to chlorine dioxide (ClO 2 ), especially the use of chlorine dioxide as a sterilizing agent. Background technique [0002] Chlorine dioxide is a strong oxidizing agent; it can undergo strong oxidation reactions with phenols, sulfur, tertiary amines, organic microorganisms, metal ions, etc.; The reaction will not produce chlorophenol, chloroform and other odorous substances and carcinogens, and it will not cause secondary pollution to the environment. In the late 1980s, chlorine dioxide was approved by the US Department of Agriculture and the US Environmental Protection Agency as a food disinfectant and drinking water disinfectant. Disinfectant is the first, widely used in sterilization, disinfection, antisepsis, deodorization, fresh-keeping and other fields, known as the fourth generation of disinfectant. [0003] In the traditional culture of ganoderma lucidum, the prepared medium needs to be sterilized by high tempera...

Claims

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Application Information

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IPC IPC(8): C12N1/14A61L2/18
Inventor 党建章
Owner SHENZHEN POLYTECHNIC