Extraction method of chlorella growth factor (chlorella extract and CGF)

A growth factor and extraction method technology, applied in the high-tech biological field, can solve the problems of low output rate, destruction of functional components, high cost, etc., and achieve the effect of high residue utilization rate, low cost and low temperature requirement

Inactive Publication Date: 2002-09-04
INST OF SOIL & FERTILIZER JIANGSU PROV ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, only a few developed countries have mastered the extraction technology of chlorella growth factor (chlorella essence, CGF) at present, and it is still blank in China.
According to the applicant's knowledge, the method of water extraction or alkaline solution extraction is mainly used in foreign countries. During the extraction process, some functional components are destroyed, resulting in poor activity of functional components, low output rate, high cost, and ineffective use of residues as waste. question

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Chlorella growth factor (chlorella essence) extraction final product of the present embodiment is chlorella essence dry powder, and its steps are as follows: A. Warm bath - put chlorella powder or chlorella mud into 50 ℃ Soak in warm water at ~70°C

[0017] For about 10 hours, expand and soften the cell wall of chlorella; B, break the wall - add pectinase to the chlorella pulp after the warm bath, and control the temperature at 40 ° C ~

[0018] 50°C for about 12 hours to hydrolyze the cell wall of Chlorella,

[0019] Form broken algae mud; C. Enzyme hydrolysis - add nuclease and protease to the broken chlorella slurry, temperature control

[0020] Made at 40°C-50°C to hydrolyze it into a mixture of chlorella essence; C.'separation——centrifuge the hydrolyzed mixture with a centrifugal device to obtain the chlorella growth factor

[0021] (Chlorella essence) liquid; D. Utilization of residues—spray-dry the separated residues and ...

Embodiment 2

[0024] The chlorella growth factor (chlorella extract) of the present embodiment is extracted and the final product is a chlorella extract capsule or tablet, and its steps are as follows: A. Warm bath——put the chlorella powder or the chlorella mud Soak in warm water at 50°C to 70°C,

[0025] Expand and soften the cell wall of chlorella; B break the wall - add pectinase and cellulase to the chlorella pulp after warm bath, temperature control

[0026] Made at 40℃~50℃, the cell wall of Chlorella can be hydrolyzed to form

[0027] into broken wall algae mud; C. enzymatic hydrolysis - add nuclease and protease to the chlorella slurry after broken wall, temperature control

[0028] Made at 40°C-50°C to hydrolyze it into a mixture of chlorella essence; C.'separation——centrifuge the hydrolyzed mixture with a centrifugal device to obtain the chlorella growth factor

[0029] (Chlorella essence) liquid; D. Residue utilization—spray-dry the separation r...

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Abstract

A method for extracting chlorella growth factor (chlorella extract) belongs to the field of biological technology and includes the steps of warm-water bath, breaking wall, enzymolysis, separation andresidue utilization. This invention adopts the method of adding enzyme to make hydrolysis extract the chlorella extract so that its temp. requirement is low and its solution is neutral. This invention also adopts the process of breaking wall and fully hydrolyzes amino acids and nucleotide, etc. contained in cell.

Description

technical field [0001] The invention relates to a method for extracting raw materials of nutraceuticals, in particular to an extraction method for chlorella growth factor (also known as chlorella essence, English Chlorella Growth Factor, CGF for short), which belongs to the field of high-tech biotechnology. Background technique [0002] Chlorella is the fastest growing plant in the world. No animal or plant can grow 4 times every 20 hours like Chlorella. This is mainly due to the growth factors of chlorella (chlorella essence, CGF). The growth factor of Chlorella (Chlorella essence, CGF) is a unique cell active substance of Chlorella, in which the hydrolyzed amino acid content reaches 38.8%, including amino acids (there are 16 kinds of amino acids, 8 of which are essential amino acids for human body), nucleic acid , The content of free nucleotides is higher than 6.8%, polysaccharides 7.4%, short-linked polypeptides, proteins, vitamins, minerals, and some "mysterious ingred...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02
Inventor 韩士群
Owner INST OF SOIL & FERTILIZER JIANGSU PROV ACAD OF AGRI SCI
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