The invention relates to nutrient-rich low temperature pressed sesame oil and a preparation method thereof. The sesame oil is prepared from the following components in parts by weight: 90 to 180 partsof black sesame, 20 to 30 parts of peanut kernel, 15 to 35 parts of walnut kernel, 10 to 20 parts of chestnut, 8 to 16 parts of sunflower seed, 10 to 20 parts of filbert, 5 to 15 parts of ophiopogonjaponicus, 3 to 9 parts of rehmannia glutinosa, 10 to 20 parts of osmanthus fragrans, 15 to 20 parts of nelumbo nucifera, and 10 to 20 parts of lonicera japonica. The preparation method comprises thefollowing steps: weighing the peanut kernel, walnut kernel, chestnut, sunflower seed, filbert, ophiopogon japonicus and rehmannia glutinosa, and preparing to obtain first mixed powder; weighing the osmanthus fragrans, nelumbo nucifera and lonicera japonica, and preparing to obtain second mixed powder; shelling the black sesame, treating the shelled black sesame, and then rolling the shelled blacksesame to be a 0.2 to 0.3mm preform in a crushing roll; isolating filter residue and crude sesame oil; obtaining finished sesame oil. The sesame oil has the advantages of rich nutrition and good effect.