Extraction method of chlorella growth factor (chlorella extract and CGF)
A growth factor and extraction method technology, applied in the field of high-tech biology, can solve the problems of low yield, destroy functional components, and high cost, and achieve the effects of high residue utilization, low temperature requirements, and cost-effectiveness.
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Embodiment 1
[0018] The chlorella growth factor (chlorella extract) of the present embodiment is extracted and the final finished product is chlorella extract dry powder, and its steps are as follows:
[0019] A. Warm bath——Put chlorella powder or chlorella mud in warm water at 50°C to 70°C for about 10 hours to expand and soften the cell wall of chlorella;
[0020] B. Wall breaking—add pectinase to the chlorella pulp after the warm bath, and control the temperature at 40°C to 50°C for about 12 hours to hydrolyze the cell wall of the chlorella to form wall-broken algae mud;
[0021] C. Enzymatic hydrolysis - adding nuclease and protease to the broken chlorella pulp, and controlling the temperature at 40°C to 50°C to hydrolyze it into a mixture of chlorella essence;
[0022] C'. Separation - use centrifugal equipment to centrifuge the hydrolysis mixture to obtain the liquid of chlorella growth factor (chlorella essence);
[0023] D. Utilization of residues—spray-dry the separated residues ...
Embodiment 2
[0027] The chlorella growth factor (chlorella essence) extraction final product of this embodiment is chlorella essence capsule or tablet, and its steps are as follows:
[0028] A. Warm bath - Soak chlorella powder or chlorella mud in warm water at 50°C to 70°C to expand and soften the cell wall of chlorella;
[0029] B broken wall - add pectinase and cellulase to the chlorella pulp after warm bath, the temperature is controlled at 40 ℃ ~ 50 ℃, so that the chlorella cell wall undergoes hydrolysis reaction, and the broken wall algae mud is formed;
[0030] C. Enzymatic hydrolysis - adding nuclease and protease to the broken chlorella pulp, and controlling the temperature at 40°C to 50°C to hydrolyze it into a mixture of chlorella essence;
[0031] C'. Separation - use centrifugal equipment to centrifuge the hydrolysis mixture to obtain the liquid of chlorella growth factor (chlorella essence);
[0032] D. Utilization of residues—spray-dry the separated residues and process the...
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