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Artificially cultured triploid Hepu nacre and its pearl culturing method

A technology of artificial cultivation of pearl oysters in Hepu, applied in the field of artificial cultivation of triploid pearl oysters in Hepu, can solve the problems of yield and quality limitation, high toxicity of CB and high price.

Inactive Publication Date: 2002-10-09
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, farmers mostly relied on experience to raise shellfish, and the yield and quality were greatly limited.
In the past, people used cytochalasin B (CB) as an inducer to induce triploids, which was relatively successful. However, CB is highly toxic, carcinogenic, and expensive, and its safety is a problem that needs to be solved.
The method of artificially cultivating triploid Hepu Pinctada induced by 6-DMAP and further raising pearls has not been reported in the literature

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 Seedling cultivation (artificially cultivated triploid Pinctadas):

[0031] Location: Weizhou Island, Beihai, Time: April 1999. Select 19 female shellfish and 9 male shellfish with complete shell bodies, obvious radiation, sharp and firm phosphorus sheets, well-developed silk, shell height of more than 6.5 cm, and full gonads. In the beaker, sieve and filter, the total number of eggs is 96 million, and quickly add the eggs to the water tank filled with 0.006% ammonia sea water, stay for 10 minutes, the water temperature is 23 ℃, then add an appropriate amount of sperm, and mix well to fertilize . When about 30% of the fertilized eggs have the first polar body, continue to induce the fertilized eggs with 6-DMAP (final concentration 50mg / L) in 10 glass tanks of 40×30×15 cm at a water temperature of 23°C. Minutes, the amount of eggs was 0.5 million / ml during treatment. Then use filtered seawater to wash the eggs twice and change the water, change the treatm...

Embodiment 2

[0032] Embodiment 2 Seedling cultivation (artificially cultivated triploid Pinctadas):

[0033] Location: Weizhou Island, Beihai, Time: March 1999, 22 female shellfish and 9 male shellfish were taken, and triploid Pearl oyster was artificially cultivated according to the same method as in Example 1. The result: triploid rate in the embryonic stage Up to 91%, 343,000 seedlings per cubic meter of water.

Embodiment 3

[0034] Embodiment 3 breeding beads:

[0035] According to the steps described in the Summary of the Invention, the artificially cultivated triploid Pinctada is cultured, wherein the Pinctada is placed in a low-hanging close culture for about 20 days, and the small pieces are first-year-old diploid. During the intercalation period, the water temperature in the sea area was 17-22°C, and the specific gravity was 1.022. The rest period is one month. The mortality rate of the triploid (3n) group was 23.3%, and that of the diploid (2n) group was 25.3%.

[0036] Pearl breeding results (6 months of cultivation): the rate of 3n pearls is 0.84 grains / shell, the rate of nucleus retention is 0.91 grains / shell, large pearls above 7.0mm and medium-sized pearls of 6.5-7.0mm account for 90.2%, and the average weight of pearls is 0.48 g / shell; the pearling rate of 2n group is 0.64 grain / shell, the nucleus retention rate is 0.68 grain / shell, large pearls and medium pearls account for 51.6%, a...

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PUM

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Abstract

A method for artificially culturing the Hepu triploid nacre includes culturing parents, gathering spermia and ova, fertilization, inducing, collecting larvae and culturing them, breeding and culturing in sea. A method for artificial culturing pearl with said Hepu triploid nacre includes pretreating female nacre, making small mentallum sheet, inserting nuclear, recovery culture, culturing pearl and picking up. Its advantages are high radio of triploid (more than 90%), high survical rate of larvae, and high granularity and quality of pearl.

Description

technical field [0001] The invention relates to a method for artificially cultivating triploid Hepu Pinctada, and further relates to a method for raising pearls with the artificially cultivated triploid Hepu Pinctada. Background technique [0002] With the development of society and the improvement of people's living standards, people pay more and more attention to high-tech agriculture, and the scientificization of breeding industry is becoming more and more important. In the past, farmers mostly relied on experience to raise shellfish, and the yield and quality were greatly limited. In the past, people used cytochalasin B (CB) as an inducer to induce triploids, which was relatively successful. However, CB is highly toxic, carcinogenic, and expensive, and its safety is a problem that needs to be solved. The method of artificially cultivating triploid Hepu Pinctada induced by 6-DMAP and further raising pearls has not been reported in the literature. content of the inventi...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 何毛贤林岳光姜卫国
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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