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Gene sequence and use of fish testicle development factor

A technology for gene sequence and fish, which is applied to the field of gene sequences expressed at the beginning of the neurulation stage of fish embryonic development, can solve problems such as time-consuming operation, and achieve the effect of changing fertility and artificially controlling sex.

Inactive Publication Date: 2003-03-12
INST OF AQUATIC LIFE ACAD SINICA
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  • Application Information

AI Technical Summary

Problems solved by technology

Sex hormone induction and genome manipulation can produce all-female offspring, but the operation is relatively time-consuming

Method used

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  • Gene sequence and use of fish testicle development factor
  • Gene sequence and use of fish testicle development factor

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Experimental program
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Embodiment Construction

[0018] 1. Extraction of total RNA from tail bud stage embryos by ultracentrifugation

[0019] Total RNA was extracted by cesium trifluoroacetic acid salt (CsTFA) density gradient ultracentrifugation, and the steps were detailed in the RNAExtraction Kit (Pharmaica) operating manual. The gibel carp embryos were incubated at 20°C in a culture medium filled with steamed water, and 1.4g of embryos at the tail bud stage were weighed, added to 8mL of extraction buffer, and homogenized. Centrifuge at 5,000×g to take the supernatant, and repeatedly aspirate the supernatant more than 10 times with a No. 7 needle. First add 7.5mL of CsTFA solution to a 16×89mm centrifuge tube, then gently add 3.5mL of supernatant. Ultracentrifuge at 125,000×g (32,000 rpm) for 16 hours at 15°C with a SW41 horizontal rotor. Dissolve the total RNA attached to the bottom of the tube with 250 μL TE after discarding all the solution. The Total RNA in the two tubes in the same period was combined in a 1.5ml ...

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Abstract

A gene sequence of testie development factor for fish has 761 bp for cDNA length, 54bp-476bp region for open reading frame, 141 coding amino acids, 53 bp for 5' non-coding region and 285 bp for 3' non-coding region. It plays important role in sex differentiation, male gonad development and reproduction regulation.

Description

technical field [0001] The present invention relates to the gene sequence initially expressed in the neurula stage of fish embryo development, and more specifically relates to a new gene testis development factor 1 ( Tdf1) (testis development factor 1) nucleotide sequence, amino acid sequence and its spatiotemporal expression pattern, the present invention also relates to the use of the gene. technical background [0002] Gender difference is the biggest difference among vertebrate individuals, which is not only manifested in the different reproductive organs between male and female individuals, but also in many aspects related to growth rate and economic traits such as body color and size. In long-term production practice, people have long found that animals of different sexes often have obvious differences in growth speed and commodity value. For example, the growth speed of female crucian carp, carp, mandarin fish, and flounder is about 30% faster...

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Application Information

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IPC IPC(8): A23K10/16A23K20/147A61K31/7088A61K48/00A61P15/08A61P43/00C07H21/04C12N15/12
Inventor 石耀华刘军桂建芳
Owner INST OF AQUATIC LIFE ACAD SINICA