Aflatoxin fast detecting apparatus and its making method

A technology of aflatoxin and detection device, which is applied in measurement devices, instruments, scientific instruments, etc., can solve the problems that can only be carried out in the laboratory, the operation procedures are complicated, and the detection time is long.

Inactive Publication Date: 2003-04-16
DALIAN PRACTICAL BIOTECH
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  • Abstract
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  • Claims
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Problems solved by technology

All there is following shortcoming in above-mentioned each method: operation procedure is complicated, can only be carried out in laboratory; Need professional personnel to operate, with special instrument and equipment, detection time is long (generally needs 3-10 days), required cost is high, and its cost In RMB 200-1000 / per sample

Method used

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  • Aflatoxin fast detecting apparatus and its making method
  • Aflatoxin fast detecting apparatus and its making method
  • Aflatoxin fast detecting apparatus and its making method

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Embodiment Construction

[0016]Now in conjunction with accompanying drawing (embodiment) the present invention is described in further detail: the right end that is coated with self-adhesive base plate 5 attaches wide about 20 millimeters of water-absorbing filter paper 1, with liquid spraying machine, will be transferred to the glue of 50-80OD / ml. Shujin—monoclonal antibody was sprayed on the polyester fiber pad 2 with a width of 7 mm, and dried at 37°C for 1 hour, and the polyester fiber pad 2 was attached to the place where the filter paper 1 overlapped by 1-2 mm; The liquid spraying machine with double nozzles with a phase interval of 10 mm sprays the aflatoxin-protein complex 31 acquired day sheep anti-mouse antibody 32 with a concentration of 1-2 mg / ml at a spraying amount of 0.7-1.5 microliters / cm Attached to a nitrocellulose membrane 3 with a width of 25 mm, dried at 37° C. for 1 hour, attached the nitrocellulose membrane 3 to the polyester fiber mat 2 overlapping 1-2 mm, and placed a 20 mm Th...

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Abstract

The invention detects the harmful metabolite produced by epiphyte, especially aflatoxin. The outline of the invention is as follows. The filter paper, polyester cellulose mat, film of pyroxylin and filter paper are pasted on the plastic long strip bed plate. A layer of colloidal gold-monoclonal antibody is spray coated on the polyester cellulose with 50-80 OD/ml. The following two materials are spray-coated on the film of pyroxylin alternately: aflatoxin-BSA compound body and ovine antibody for anti the immunoglobulin G1 antibody of little white rat. Both of their concentration is in 1-2 mg/ml. Both of application doses is in 0.7-1.5 microlitre/cm.

Description

technical field [0001] The invention relates to the detection of metabolites produced by fungi, especially the rapid detection of aflatoxins. Background technique [0002] As we all know, aflatoxin is a highly toxic substance and a strong carcinogen. Its production and pollution are mostly carried out through feed, grain and food. It is quite harmful to humans and animals. Therefore, scientists have paid great attention to it, and many measures have been taken to suppress aflatoxin, prevent its spread, and avoid human and animal poisoning. The qualitative and quantitative detection of its presence in feed and grain is an important part of the measures. The known method of aflatoxin detection mainly contains thin-layer chromatography (seeing the 01 phase of Chinese Health Inspection Magazine, 1998), enzyme-linked immunoassay (seeing Indian Veterinary Journal (1997, 74 phase)) and liquid chromatography ( See Journal Chromatography, Issue 04, 1995). Thin-layer chromatograph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/577
Inventor 林祥
Owner DALIAN PRACTICAL BIOTECH
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