Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Trans-species transfer of apoptotic genes and transgenic plants developed thereby

A technology of transgenic plants and cells, which is applied in the field of modulating plant cell apoptosis, can solve problems such as uncertain relations at best, and achieve the effects of extending shelf life, improving viability, and increasing crop yield

Inactive Publication Date: 2003-04-23
UNIV OF NEBRASKA LINCOLN
View PDF49 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Interestingly, efforts to identify similar pathways in plants have not been fruitful, and the relationship of mammalian and invertebrate apoptosis to plant programmed cell death pathways is uncertain at best

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Trans-species transfer of apoptotic genes and transgenic plants developed thereby
  • Trans-species transfer of apoptotic genes and transgenic plants developed thereby
  • Trans-species transfer of apoptotic genes and transgenic plants developed thereby

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Embodiment I

[0165] The following examples are intended to illustrate rather than limit the invention. Examples Example I Construction of a binary vector containing IAP

[0166]The open reading frame (ORF) in the vector pOPIAPRC (Birmbaum et al., J. of Virology 68:2521-2528, 1994) was modified by PCR to introduce the NcoI site at the 5'end and the BamHI site at the 3'end point. This operation introduces an Ala residue between the Met (position 1) and Ser (position 2) residues of the natural protein. The PCR conditions used were as follows: 1 minute at 94°C; 1 minute at 45°C; 2 minutes at 72°C for 2 cycles, followed by another 1 minute at 94°C; 1 minute at 55°C; and 2 minutes at 72°C for 35 cycles. Primer prc-5: 5'-gttgcagaccatggccagctcccgagcattggc-3'. Primer pre-3: 5'-ttttggatccttttattgttacacttgg-3'

[0167] The PCR product was digested with NcoI and BamHI and subcloned into the plant expression cassette pRTL2 (described as pRTLGUS by Carrington et al., Journal of Virology 64:1590-1597, 1990)....

Embodiment II

[0167] The PCR product was digested with NcoI and BamHI and subcloned into the plant expression cassette pRTL2 (described as pRTLGUS by Carrington et al., Journal of Virology 64:1590-1597, 1990). The resulting vector was called pPTN144 (Figure 1A). The 35S-IAP cassette of pPTN144 was subcloned into the binary vector pZP212 using HindIII (Hajdukiewicz et al., Plant Mol. Bio. 25:989-994, 1994). The resulting vector was called pPTN 148 (Figure 1B). Example II Construction of a binary vector containing Ced-9

[0168] The ORF of Ced-9 was modified by PCR to introduce the NcoI site at the 5'end and the XbaI site at the 3'end. This operation introduces an Ala residue between the Met (position 1) and Thr (position 2) residues of the natural protein. The PCR conditions are the same as those used to transform the pOPIAPRC ORF. Primer ced-5: 5'-gaattccggtttgagccatggcgacacgctgcacggcg-3' primer ced-3-2: 5'-ttttttctagaaatacgttacttcaagctg-3'

[0169] The PCR product was digested with NcoI and Xb...

Embodiment III

[0169] The PCR product was digested with NcoI and XbaI and subcloned into the plant expression cassette pRTL2 (Carrington et al., Journal of Virology 64:1590-1597, 1990). The resulting vector was called pPTN143 (Figure 3A). The ced-9 plant expression cassette of pPTN143 was subcloned as a HindIII fragment into the binary vector pZP212 (Hajdukiewicz et al., Plant Molecular Biology 25:989-994, 1994). The resulting vector was called pPTN 147 (Figure 3B). Example III Construction of a binary vector containing Bcl-2

[0170] The bcl-2 cDNA was modified to introduce the NcoI site at the 5'end of the bcl-2 ORF and the XbaI site at the 3'end of the ORF. Using the same conditions as in Example I, the PCR reaction was carried out with the following primers: primer Bcl2-5: 5'-tttttcctctgggagggccatggcgcacgctgg-3' primer Bcl2-3: 5'-ttttttctagatgctcttcgggcgtgg-3'

[0171] This operation introduces Ala residues between the Met (position 1) and His (position 2) residues of the natural protein. Th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to the cross-species transfer of apoptosis genes to plant cells, the transgenic plants produced thereby, and screening assays using these plants. The present invention also relates to drug discovery screening methods utilizing transgenic plant cells. In addition, the present invention relates to methods for identifying components of the apoptotic pathway in plant cells using non-plant proteins and nucleic acids.

Description

Technical field [0001] The present invention generally relates to modulation of plant cell apoptosis, and more specifically to a new gene transfer, that is, the transfer of genes encoding apoptotic pathway proteins from non-plant species to plants, resulting in higher levels of resistance to a variety of biological and non-biological attacks. Resistant plants and provide other related advantages. Background of the invention [0002] Recently, people have gradually deepened their understanding of the complex signal transduction pathways that induce apoptosis in animal cells, and have begun to work on identifying organisms with different levels of evolution, such as similar pathways in plants. It is believed that the apoptosis-mode of plants only occurs at specific points in the development process, and the response of plants to pathogens can trigger apoptosis (see Woodson et al., Plant Physiology, 99:526-532, 1992; O 'Neill et al., Plant Cell, 5:419-432, 1993; Chasan, Plant Cell, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47C12N5/10C12N15/09C12N15/82C12Q1/68A01H5/00C12R1/91
CPCC12N15/8271C07K14/4747C12N15/8282C12N15/8257C12N15/8283C12N15/8263C12N15/8279C12N15/8266
Inventor M·B·迪克曼
Owner UNIV OF NEBRASKA LINCOLN
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com