Water soluble heteropolysaccharide with anti-tumor activity and its preparing method and use

An anti-tumor activity, water-soluble technology, applied in the field of molecular biology, can solve the problems of difficult storage and transportation, difficult to popularize pachyrhiza, long cycle, etc., achieve significant anti-tumor activity, obvious anti-tumor activity, and simple preparation method Effect

Inactive Publication Date: 2003-06-25
WUHAN UNIV
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Problems solved by technology

However, due to the long period of artificial cultivation of Poria cocos, and it is difficult to pr...

Method used

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Embodiment

[0015] The strains of Poria cocos were provided by the Culture Collection Center of the Institute of Microbiology, Chinese Academy of Sciences, No. 5.78 (the public can buy them at any time). The strains were first cultured on slant media. The test tubes and cotton plugs used for culture are first sterilized at 121°C for 30 minutes (empty sterilization), then put into the culture medium and sterilized at 121°C for 30 minutes, placed on an oblique cooling to make it solidify into a slope, and then inoculated in a sterile room . The slant culture was carried out at a constant temperature of 28°C for a period of 7 days. The liquid medium formula is as follows: 1L distilled water contains corn steep liquor (10mL), D-glucose (25g), yeast extract (3.2g), KH 2 PO 4 (1.0g), CaCl 2 2H 2 O (0.06g), MnCl 2 4H 2 O (5 mg), MgSO 4 ·7H 2 O (0.5g), ZnCl 2 (4 mg), Fe 2 (SO 4 ) 3 (5mg), vitamin B 1 (0.1 mg). Shake flasks (250 mL) and cotton plugs used for culture were sterilized ...

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Abstract

The present invention provides a water soluble tuckahoe mycelium hetero polysaccharide with anti-tumor activity. By using corn slurry as main component to prepare culture medium, tuckahoe, mycelium is cultured through deep fermentation of wild Tuckahoe strain. Water soluble hetereo polysaccharide is extracted from the mycelium with physiological saline and hot water. The hetero polysaccharide consists of alpha-D-glucose, mannitol, galactose and protein in different content. Experiments shows that the hetero polysaccharides can inhibit the growth of Sarcoma 180 tumor in mouse and acute leukovirus cell, so that they may be used as antitumor medicine and health article for raising body's immunological function.

Description

technical field [0001] The invention relates to a water-soluble heteropolysaccharide with antitumor activity, a preparation method and application thereof. It belongs to the field of natural polymers and also belongs to the field of molecular biology. Background technique [0002] In the 1970s, due to the progress made in the research of chemical functions of cell membranes, immune substances and new drugs, as well as the discovery of complex polysaccharides such as glycoproteins and glycolipids, polysaccharides have been convinced that polysaccharides have many biological activities. The research of Chihara (J.Immunol.Immunopharmacol., IV, 85, 1983) believes that the activity of polysaccharides is not directly killing cells as in chemotherapy, but there is an indirect anti-tumor activity, that is, it is activated by the immune system. The delivered activity is primarily a stimulation of specific non-specific or specific functions of the immune system and therefore has mini...

Claims

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Application Information

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IPC IPC(8): A61K31/715C08B37/00C12P19/04
Inventor 张俐娜陈莉金勇林雨露张玫张志强
Owner WUHAN UNIV
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