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Specificity determination and application of one section of candida albicans DNA sequence

A technology of Candida albicans, DNA sequence, applied in the field of biomedicine

Inactive Publication Date: 2003-08-20
昆明寰基生物芯片开发有限公司
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Problems solved by technology

[0002] Candida albicans is a pathogen that frequently infects women's vagina. Accurate diagnosis is the basis for immediate and reliable treatment. At present, it can only be diagnosed according to clinical indications

Method used

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  • Specificity determination and application of one section of candida albicans DNA sequence
  • Specificity determination and application of one section of candida albicans DNA sequence
  • Specificity determination and application of one section of candida albicans DNA sequence

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Embodiment Construction

[0019] Firstly, the nucleotide sequence of Candida albicans was searched in the gene database with Candida albicans as the keyword. A total of several closely related nucleotide sequences were obtained, and these nucleotide sequences were compared with the nucleotide sequences (nr) in the gene database using the Blast online software on the NCBI network to exclude non-specific nucleotide sequences, A 1 Kb specific nucleotide sequence was obtained. More than 20 pairs of PCR primers were designed based on this sequence, DNA was extracted from clinical specimens, PCR amplification was performed, and a pair of sequences and primers suitable for PCR amplification were screened out. The products obtained by PCR amplification were consistent with the expected size (see Fig. 1. The serial numbers in the figure are: 1. Standard molecular weight DNA, the molecular weights from top to bottom are: 622, 527, 404, 309, 242 / 238, 217, 201, 190, 180, 160+160, 147+147, 123; 2.4.5 Clinical nega...

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Abstract

The present invention relates to specificity determination and application of one section of candida albicans DNA sequence, and belongs to the field of biomedicine. Through gene data base data search and analysis, the specificity and PCR amplification primer of one section of candida albicans DNA sequence is determined, and the sequence length is 241 bp. By means of PCR amplification and cloning of women's genital tract candida albicans infection sample, the sequence is obtained and sequenced to obtain the result the same as the predicted. Biological informatics and gene chip hybrid analysis proves the specificity of the sequence to candida albicans. The present invention is used as probe and PCR amplification detection sequence for gene chip diagnosis for candida albicans.

Description

Technical field: [0001] The invention relates to the specificity determination and application of a Candida albicans DNA sequence and belongs to the technical field of biomedicine. Background technique: [0002] Candida albicans is a pathogen that frequently infects women's vagina. Accurate diagnosis is the basis for immediate and reliable treatment. At present, it can only be diagnosed according to clinical indications. PCR and gene chip diagnosis is a fast and accurate detection method. Diagnostic methods of this type rely on the availability of specific nucleic acid sequences. Invention content: [0003] The invention is to determine a section of candida albicans specific nucleotide sequence and its PCR primer through bioinformatics and molecular biology techniques. The sequence and corresponding PCR primers can be used to establish gene chip and PCR methods to diagnose Candida albicans. [0004] The technical scheme of the present invention is: the present invention...

Claims

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Application Information

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IPC IPC(8): C12N15/31C12Q1/68
Inventor 谭德勇朱宝生
Owner 昆明寰基生物芯片开发有限公司
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