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Multiplication and in vitro flowering of rose cultivars

A technology of Rosa and Rosa, applied in the fields of plant cultivation, plant asexual reproduction and horticulture

Inactive Publication Date: 2003-11-12
INST OF MOLECULAR AGRO BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Improved Blaze, the medium used for climbing roses, resulted in significant growth of three miniature rose species, but not all hybrid tea roses (Hasegawa, J. Amer Soc. Hort. Sci. 1980, 105: 216-220)

Method used

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  • Multiplication and in vitro flowering of rose cultivars
  • Multiplication and in vitro flowering of rose cultivars
  • Multiplication and in vitro flowering of rose cultivars

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] In this example, adding thidiazuron to the culture medium can enhance the induction of flower buds. Young shoots with axillary buds from mature plants were used as explants. The Rosa explants were placed on a solid medium and cultured under light conditions for 16 hours, and the light intensity was 4500-5500 lux (the light source was provided by a fluorescent lamp). The temperature was 23°C when there was light, and 19°C when there was no light. Whole shoots were cultured on RMS1 medium, the explants gradually turned green, and usually sprouts formed after 10-15 days. Cut the young shoots from the young shoots. Put the propagated seedlings (reproduced more than 2 times) into the RMS 2 medium culture to induce flower buds. After induction of flower buds, plant seedlings are transferred to elongation medium for in vitro flowering. RMS in elongation medium 4 Cultivate the seedlings in the medium for 15-30 days, and then transfer them into MS containing ampicillin at ...

Embodiment 2

[0066] In this example, adding zeatin to the medium can enhance the induction of flower buds. Young shoots with axillary buds from mature plants were used as explants. The Rosa explants were placed on a solid medium and cultured under light conditions for 16 hours, and the light intensity was 4500-5500 lux (the light source was provided by a fluorescent lamp). The temperature was 23°C when there was light, and 19°C when there was no light. Put the propagated seedlings (reproduced more than 2 times) into the RMS 3 medium culture to induce flower buds. After induction of flower buds, directly transfer the seedlings with flower buds into MS containing 20-50mg / L ampicillin 0 make it bloom. Flowering will occur within 15-20 days. 40-50% of the plant seedlings can grow flower buds, and more than 80% of the flower shapes are normal. Unflowered seedlings can be returned to continue breeding. In an environment lower than 25°C, the flowering period of the cultured flowers exceeds...

Embodiment 3

[0068] Plants of Rosa cultivars Holiday PARADE®, Bright Red PARADE®, Bianca PARADE®, Frost White PARADE® were used as explants and grown under the above-mentioned composition and method. Propagation medium RMS5 containing indoleacetic acid instead of naphthaleneacetic acid replaced RMS 1 . More than 30% of the seedlings are propagated, and plant seedlings with normal flower shape can be obtained through the method of in vitro flowering of the present invention.

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Abstract

The present invention is directed to compositions and methods of micropropagation of a rose plant and in vitro flowering of the rose plant. Young shoots are induced to produce buds in an enclosed vessel containing a first culturing meidum comprising benzyladenine, an auxin and 2% sucrose as carbon source. Buds are excised and cultured on a medium for propagation and multiplication of plantlets. Plantlets are then transferred to a medium comprising thidiazuron, an auxin and myo-inositol for induction of flower buds, followed by culture on a medium comprising benzyladenine and an auxin for plantlet elongation and finally culture in the fifth medium without phytohormone for flowering. Alternately, after propagation and multiplication, plantles are transferred to a medium comprising zeatin, auxin and myo-inositol for induction of flower buds followed by culture on a medium without phytohormone for elongation and flowering.

Description

technical field [0001] The invention relates to the fields of plant cultivation, plant asexual propagation and horticulture. The present invention particularly relates to the vegetative propagation of plants of the genus Rosacea (rose) or tissue cultures, and the in vitro induction of flowering of young plants of the genus Rosacea derived from plants of the genus Rosacea or tissue cultures. More specifically, the present invention relates to media formulations and effective methods for in vitro micropropagation, flower bud induction, and flowering of roses and plants of the genus Rosa. technical background [0002] Rosaceae belong to the family Rosaceae (Rosaceae Juss). The family is very large, its members include more than 100 genera and 2000 herbaceous and woody species. Many important food and ornamental plants belong to the Rosaceae family, such as strawberries, apples, apricots, cherries, pears, and black plums. The exact number of species of Ros...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008A01H4/002
Inventor 洪焰袁梅芳王光远
Owner INST OF MOLECULAR AGRO BIOLOGY
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