Extraction of saponin from seeds of trigonella foenum-graecum

A technology of fenugreek and saponin, which is applied in the field of saponin extraction, can solve the problems of large solvent consumption and unsuitability for industrial production, and achieve the effect of reducing costs

Inactive Publication Date: 2003-11-19
何昌义
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The above-mentioned method for extracting total saponins consumes a large amount of solvent, and three to four solvents are used in turns, which is not suitable for industrial production

Method used

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  • Extraction of saponin from seeds of trigonella foenum-graecum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: 1500g of degummed and degreased powder residue, add 6 times of water, add 22.5g each of mannase, amylase, and cellulase to the mixture, keep it warm at 45°C for 30 hours, and stir slowly (80 rpm) , after the completion of the reaction, centrifugal separation. The solid residue is protein. Put water on the column (type 101 large-pore resin column), wash with water, and elute with alcohol. After recovery of alcohol, 30.5 g of saponin crude product was obtained, and 17.5 g of pure product was obtained by methanol recrystallization once (MP152-155°C).

Embodiment 2

[0018] Embodiment 2: degumming, defatting fenugreek powder 1500g, add 6 times of water, add galactase, saccharification, cellulase, lipase each 30g in the mixture, at 50 ℃ for 28 hours, stir (100 rpm minutes), the reaction is completed, and the aqueous solution is centrifuged and put on the column (101 type large-pore resin column). Elute with ethanol, collect the ethanol solution, and recover the alcohol to obtain 41g of crude total saponins, and recrystallize once from methanol to obtain about 22.5g of pure product (MP: 152-155°C)

Embodiment 3

[0019] Example 3: 1500 g of degummed and degreased fenugreek powder, add 6 times of water, add 10 g of lipase and cellulase in the mixture, incubate at 50° C. for 30 hours, stir at 80 rpm, and after the reaction is completed, Centrifuge, put the water liquid on the column (101 type large-pore resin), wash with water, elute with alcohol, collect the eluent, recycle the alcohol, evaporate to dryness to obtain about 25g of crude saponin, and recrystallize with methanol to obtain 10.8g of pure saponin ( MP: 152-155°C).

[0020] The above-mentioned embodiment can obtain protein 400-500g when saponin is separated. Its content is 54-58%, which can be used in food and seasoning after hydrolysis.

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Abstract

A process for extracting saponin from the seeds of common feungreek includes such steps as degumming, defatting, pulverizing, extracting in the aqueous solution containing active substances, such as seminase, galactase, beta-amylase, cellulase, etc. at 40-80 deg.c for 20-48 hr while stirring, and adsorbing by macroreticular resin. Its advantages are low cost and high output rate (0.8-1.5%).

Description

technical field [0001] The present invention relates to a method for extracting saponins, in particular to a method for extracting saponins from fenugreek seeds which are successfully extracted from fenugreek seeds by utilizing the residue after desugaring and degreasing from fenugreek seeds and using an aqueous solution mixed with active substances . Background technique [0002] Fenugreek, also known as Lubazi, is the mature seed of an annual leguminous herb. It is warm in nature, bitter in taste, and has the effects of warming kidney yang and dispelling cold and damp. Recently, fenugreek has been used to treat type II diabetes and has achieved good results. Long-term use by diabetics has not been found to have toxic effects on liver and kidney. my country's Pharmaceutical Bureau has included fenugreek as one of the medicinal materials for health care products. [0003] Fenugreek is not only used as medicine, but also can be used for food, feed and industrial purposes....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J71/00C07J75/00
Inventor 何昌义蒋保民何幼鸾吴秀莲田长城
Owner 何昌义
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