Spinous spheric structure of coprinus comatus hypha and its application

A hair-head ghost umbrella and spine-shaped technology, applied in the field of spine-shaped ball structures, can solve problems such as endangering human health, groundwater pollution, destroying ecological balance, etc., and achieve the effects of short cultivation time, low cost, and strong poisoning effect.

Inactive Publication Date: 2004-02-04
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the advancement of science and technology, studies have found that most chemical nematicides are not only highly toxic to kill natural enemies, destroying ecological balance and endangering human health, but also seriously pollute groundwater

Method used

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  • Spinous spheric structure of coprinus comatus hypha and its application
  • Spinous spheric structure of coprinus comatus hypha and its application
  • Spinous spheric structure of coprinus comatus hypha and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: The spinous ball structure of Coprinus vulgaris LHA-7

[0038] Sterilize a petri dish with a diameter of 9cm and pour 15ml of PDA agar medium into it.

[0039] Inoculated on the medium and cultivated at 24°C. After 2 days, the mycelium morphology was studied with an optical microscope every day. The results showed that the obvious spine-like globule structure was found on the hypha from 2 days, and then as time passed, the spine-like globules continued to increase, and reached a peak after 5 days.

[0040] The morphological characteristics of spinous globules were studied by conventional scanning electron microscopy (SEM) techniques. The LHA-7 strain was inoculated on a PDA agar plate and cultured at 24°C for 5 days. Take the agar block (8mm×8mm) that has produced a large number of spiny pellets near the edge of the colony, and immediately put it in 2.5% glutaraldehyde fixative solution at 4°C for 24 hours, and then use 0.2M phosphate buffer (pH7.2 ) Wash three t...

Embodiment 2

[0041] Example 2: Preparation of mycelium containing spiny globules

[0042] Sterilize a petri dish with a diameter of 9 cm and pour it into PDA agar medium, inoculate Coprinus pilosa LHA-7 on the medium, and cultivate it at 24°C. Obvious spine-shaped globules were found on the hypha from 2 days, and then as time passed, the spine-shaped globules continued to increase, and reached a peak after 5 days. At this time, the mycelium has a very good toxic effect on peanut root knot nematode and P.redivivus nematode, and the lethality of nematode reaches more than 95% within 8 hours.

Embodiment 3

[0043] Example 3: Separation and purification of spiny pellets

[0044] Fold the 12 layers of lens cleaning paper and place it in a glass funnel with a diameter of 10 cm as a filter medium, soak it with distilled water and make it closely adhere to the inner wall of the funnel, and then wrap the entire funnel with tin tissue paper for routine sterilization backup use.

[0045] First, according to Example 2, the LHA-7 mycelium that produces a large amount of spinous globules was cultured. Then add 7ml sterile water to the petri dish, gently scrape the surface with a glass scraper for 2 minutes, suck out the liquid from the plate with a pipette, add it to the above sterile funnel and filter, collect the filtrate with a sterile 1.5ml centrifuge tube . Centrifuge the centrifuge tube containing the filtrate at 12,000 rpm for 5 minutes, discard the supernatant, and wash the precipitate with sterile water; centrifuge again (10,000 rpm for 3 minutes), discard the supernatant, and use the ...

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Abstract

A spinous globule of Coprinus comatus LHA-7 (CGMCC No.0932) and its activity for killing namatode are disclosed. It is especially used to kill the carcithium namatode and saprogenic namatode of peanut with the effect of 95-95.8%.

Description

Technical field: [0001] The invention relates to a spinous globule structure of Coprinus pilosa hyphae and its application, belonging to mycology and biological pesticides [0002] Technical field. Background technique: [0003] Coprinus comatus, commonly known as Coprinus comatus, has a stem like a chicken leg and a ball-like top. It is dark yellow and dark brown when dried. It belongs to the kingdom of Fungi, Basidiomycota, Layer Fungi, Agaricus, Coprinus and Coprinus. It is a kind of edible fungus that can be cultivated artificially. Coprinus comatus is not only tender and delicious in meat quality, rich in nutrition, but also has therapeutic effects such as replenishing the spleen and stomach, relieving the mind and nerves, and lowering blood sugar. According to the analysis report, every 100 grams of dried mushrooms contains 25.4 grams of crude protein, 3.3 grams of fat, 58.8 grams of total sugar, 7.3 grams of fiber, 12.5 grams of ash, and a caloric value of 346 kcal. Contai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04A01N63/00C12N1/14
Inventor 张克勤罗宏周薇
Owner YUNNAN UNIV
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