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A kind of Serratia marcescens ps-1 bacterial strain isolated from flea beetle and its application

A technology of Serratia marcescens, PS-1, applied in the directions of application, bacteria, microorganism-based methods, etc., can solve the problems of inconvenient pest control, inability to achieve both common pest control, etc., to ensure yield and quality, sustainable control effect

Inactive Publication Date: 2015-07-29
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
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Problems solved by technology

[0007]The pathogenic strain of Serratia marcescens was also isolated from the larvae of beet armyworm [Qi Fangjun, Liu Ying, Ji Zhiying. A new species of beet armyworm Isolation and identification of pathogenic bacteria. Acta Plant Protection, 2004, 31(2): 223-224; Yang Qiong, Zhou Yu, Han Guangjie, Fang Jichao. Isolation, Identification and Preliminary Analysis of Insecticidal Components of Two Beet Spodoptera Pathogenic Bacteria. Journal of Jiangsu Agricultural Sciences, 2012, 28(6): 1267-1271]. Serious pest, but so far, there has not been any report of entomopathogenic microorganisms - Serratia marcescens infecting the flea beetle, let alone the slime directly isolated from the flea beetle Serratia pathogenic strains related technical reports, and the technical research of Serratia marcescens pathogenic strains can also make flea beetle pathogenicity is still blank in China, and it is impossible to realize the simultaneous treatment of two common pests. The prevention and control of pests in agricultural production brings great limitations and inconvenience

Method used

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  • A kind of Serratia marcescens ps-1 bacterial strain isolated from flea beetle and its application
  • A kind of Serratia marcescens ps-1 bacterial strain isolated from flea beetle and its application
  • A kind of Serratia marcescens ps-1 bacterial strain isolated from flea beetle and its application

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Experimental program
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Effect test

Embodiment 1

[0038] The isolation culture method of embodiment 1 Serratia marcescens PS-1

[0039] 1. Isolation and re-infection of pathogenic bacteria

[0040] S1. Isolation of pathogenic bacteria

[0041] Take flea beetle larvae that were naturally infected with Serratia marcescens and died of red body color, and inoculated them into flat LB agar medium (agar 15-20g, sodium chloride 10g, tryptone 10g, yeast extract powder 5g , water 1L, pH 7.2), and cultured upside down in a 30°C incubator. After colonies formed, pick dark red colonies and separate them by plate streaking. Repeat 5 to 7 times to obtain a single colony, and then transfer to Put it on the LB agar medium on the slant of the test tube, and store it in the refrigerator at 4 °C.

[0042] S2. Re-infection of pathogenic bacteria

[0043] Inoculate the isolated single colony strain into a triangular flask filled with 100ml LB liquid medium (peptone 10g, yeast extract powder 5g, sodium chloride 10g, water 1L, pH7.2), and plac...

Embodiment 2

[0056] Example 2 The biological activity of Serratia marcescens PS-1 strain to flea beetle larvae

[0057] The flea beetle adults of the tested insect source were collected from the plant protection farm of the School of Resources and Environment, South China Agricultural University (the source of the tested insect is not limited to the present invention, and other sources of insect sources are also applicable to the present invention), and it was placed in the net room The planted cabbage seedlings were raised, and the eggs of the flea beetle were collected. After the larvae hatched, they were raised in petri dishes with radish slices until the third instar for testing.

[0058] Pathogen culture Pathogens were inoculated in 100 ml LB liquid medium, placed on a constant temperature shaker at 30°C, activated at 140 r / min for 10 hours, then transferred 1 ml of the bacterial liquid to 100 ml LB liquid medium, at 30°C, 140 r / min Cultivate for 24h. After centrifugation at 5000...

Embodiment 3

[0067] Example 3 Toxicity determination of Serratia marcescens PS-1 strain to flea beetle adults

[0068] The cultivation of Flea beetle and pathogenic bacteria for testing is the same as in Example 2.

[0069] Test method: Pick 1.5g of washed and dried Chinese cabbage leaves, 8 cfu ml -1 Soak in the bacterial suspension (containing 0.1% Tween-80) for 30 s, put it in a ventilated place to dry, put it in a petri dish, cover it with a plastic cover (9 cm in diameter, 21 cm in height), and insert it into yellow rice that has been starved for 2 hours. There were 10 flea beetle adults, and after 24 h, they were replaced with fresh cabbage leaves to continue feeding. The leaves of Chinese cabbage were treated with double distilled water containing 0.1% Tween-80 as the control group. Each treatment was repeated 5-7 times. Observed for 12 days, and recorded the number of dead adults every day. The test is carried out under the conditions of a temperature of 25±2°C, a relative hu...

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Abstract

The invention discloses a Serratia marcescens PS-1 strain isolated from Flea beetle flavus and application thereof. The present invention directly isolates a strain of Serratia marcescens (Serratia marcescens) strain from the body of the diseased flea beetle larva for the first time, which was preserved in the General Microbiology Center of China Microbiology Culture Collection Management Committee on July 22, 2013. The number is CGMCC No.7948. The Serratia marcescens PS-1 strain has strong pathogenicity to the larvae and adults of the flavonoid beetle and the larvae and adults of the beet armyworm, and can effectively control the development of the experimental population of the beet armyworm. The invention provides a technical basis for the field population control of the flea beetle and the beet armyworm, and is important for safely, effectively and sustainably controlling the flea beetle and the beet armyworm, and effectively ensuring the yield and quality of vegetables practical significance and practical value.

Description

technical field [0001] The invention relates to the field of research and application of microbial insecticides for crop pests, and more specifically relates to a Serratia marcescens PS-1 strain isolated from flea beetle larvae and its application. Background technique [0002] Yellow curved flea beetle Phyllotreta striolata (Fabricius) is a worldwide pest of cruciferous vegetables [Smith E H. Revision of the genus Phyllotreta Chevrtolat of America north of Mexico. Part 1. The maculate species (Coleoptera: Chrysomelidae, Alticimae). Fieldiana (Zool), 1985, 28: 1-38; Investigation and analysis of A population dynamics. Plant Protection, 2000, 26 (4): 1-3; Song Yanxia, ​​Dong Yizhi, Zhang Maoxin. Research on the value loss and control indicators of Chinese cabbage caused by flea beetle damage. Journal of South China Agricultural University. 2011 (1): 53-56]. In recent years, with the continuous expansion of the planting area of ​​cruciferous vegetables, the occurrence an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P7/04C12R1/43
Inventor 凌冰杨建云张茂新
Owner SOUTH CHINA AGRI UNIV
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