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Embedding material biological sample and method for making specimen of human body

A technology of biological specimens and embedding agents, applied in the preservation, application, and animal husbandry of human or animal bodies, which can solve the problems of unfavorable observation of human tissue, increased cost, and increased weight, so as to facilitate the observation of human tissue and reduce production costs. Effect of cost and manufacturing cost reduction

Inactive Publication Date: 2004-03-10
王孔宝
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Problems solved by technology

However, the human specimens made by this method also have the following disadvantages: 1. The embedding layer is yellow in varying degrees and has poor transparency, which is not conducive to observing human tissues and affects its use value; There is a phenomenon of separation between the embedding layer and the human body specimen, and it is easy to form a mist between the embedding layer and the human body specimen, so that the specimen cannot be seen clearly; Separation phenomenon, in order to ensure the strength of the embedding layer, the thickness of the embedding layer must be increased, which increases the amount of embedding agent used, which increases the manufacturing cost and increases the weight; 4. Since there is no special treatment for the mold, the embedding of the specimen The outer surface of the layer is rough, and the outer surface needs to be ground and polished, the process is complicated, and the cost increases

Method used

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  • Embedding material biological sample and method for making specimen of human body
  • Embedding material biological sample and method for making specimen of human body
  • Embedding material biological sample and method for making specimen of human body

Examples

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Effect test

Embodiment 1

[0046] This embodiment is a biological specimen embedding agent, which includes epoxy resin, curing agent and diluent. Wherein, the epoxy resin is epoxy resin E40, the curing agent is polysulfide rubber, and the diluent is n-butyl glycidyl ether. The volume ratio of epoxy resin, curing agent and diluent is:

[0047] Epoxy Resin Hardener Thinner

[0048] 1 0.01 0.1

Embodiment 2

[0050] This embodiment is a biological specimen embedding agent, which includes epoxy resin, curing agent and diluent. Among them, the epoxy resin is epoxy resin E40, the curing agent is ATU adduct, and the diluent is allyl glycidyl ether. The volume ratio of epoxy resin, curing agent and diluent is:

[0051] Epoxy Resin Hardener Thinner

[0052] 1 0.01 0.1

Embodiment 3

[0054] This embodiment is a method of using a biological specimen embedding agent to make a human specimen, and the method adopts the following steps:

[0055] a. Slicing:

[0056] First, the human corpse is treated with formaldehyde solution for anticorrosion and fixation. After the anticorrosion and fixation treatment, the whole body is prepared for skin, and then the corpse is rinsed. Then the corpse is frozen at a temperature below -25°C. After freezing through, the corpse is sawed into the required thickness. slice; finally, the two sections of the slice are planed so that the thickness of the slice is 10mm;

[0057] b. Cleaning, washing:

[0058] Thaw the slices obtained in step a, remove the contents in the lumens such as intestinal lumen and blood vessels, and wash them;

[0059] c. Dehydration:

[0060] First, immerse the slices treated in step b into 70%, 80%, 90%, absolute alcohol successively for 4 stages of gradient dehydration, and the time of each stage of de...

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Abstract

An embedding medium for biological specimen is prepared from epoxy resin E40, solidifying agent and diluent in volume ratio of 1:(0.01-0.05):(0.1-0.2). A process for preparing human body specimen includes incising, removing impurities, flushing, dewatering, immersing, moulding, embedding and demoulding. Its advantages are high transparency and no separation of specimen from embedding medium.

Description

Technical field: [0001] The invention relates to an embedding agent for embedding biological samples and a method for making human body samples using the embedding agent. Background technique: [0002] At present, the preservation method of biological specimens mainly adopts the traditional wet preservation method, that is, the biological specimens are soaked in preservatives for storage. The more commonly used preservative is formaldehyde. First, the formaldehyde is formulated into a 10% aqueous solution, and then the biological specimen is soaked in the formaldehyde aqueous solution. Because formaldehyde has a strong pungent smell, it can cause serious damage to the human body's respiratory, digestive, nervous and visual systems, and has carcinogenic effects. Stored in a sealed container, it takes up a lot of space and is inconvenient to carry and move. Also in the process of preparing formaldehyde aqueous solution and making biological specimens, in order to avoid conta...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 王孔宝史为栋杨成杰
Owner 王孔宝
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