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Method for fast detecting marker of hepatitis b virus with accuracy

A hepatitis B virus and marker technology, applied in measurement devices, resistance to vector-borne diseases, instruments, etc., to achieve the effect of strengthening detection quality, preventing operation errors, and ensuring accuracy

Inactive Publication Date: 2004-08-11
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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Problems solved by technology

[0004] The purpose of the present invention is to develop a computer processing method for detecting hepatitis B virus markers with a microplate reader aiming at the defects in the current hard judgment and manual interpretation and making report sheets. This method is easy to operate and standard in adding samples. , automatic printing, time-saving and labor-saving, very accurate test results, eliminating false negatives, false positives, missed detections, wrong detections, etc., fast detection speed, network retrieval and real-time quality control, etc.

Method used

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  • Method for fast detecting marker of hepatitis b virus with accuracy
  • Method for fast detecting marker of hepatitis b virus with accuracy

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Embodiment Construction

[0024] The present invention will be described in further detail below in conjunction with the accompanying drawings, please refer to the accompanying drawings. The present invention gets 92 specimens and detects, and method is carried out by following 10 steps:

[0025] The first step is to control the detection indicators of the microplate reader: turn on the power, connect the computer and the microplate reader together through the RS232 interface, and select the new sample detection set by the computer (if you want to browse and review the results of the previous detection work , then select historical data browsing), input the detection date, and operate the microplate reader to detect the hepatitis B virus markers by enzyme immunoassay. One of the indicators (if the number of specimens is less than or equal to 96, only 5 enzyme-labeled reaction plates are required, if more than 96 and less than or equal to 192, then 10 enzyme-labeled reaction plates are required, and so ...

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Abstract

The invention includes following steps: computer controlled testing of enzyme marking device; receiving result data tested by the device; recognizing OD value; saving data of tested result data to file; under region of gray scale set, determining negative or positive and saving them to database; storing quality control value and comparison value to database. The invention provides following functions: displaying all results of samples at a time; checking tested results; maintaining results and printing out tested report form including negative or positive and OD value of each sample at one time. Comparing with current method, the invention possesses features of simple operation, saving half time and labor, reflecting degree of state of an illness and infection. The invention provides online searching and real time quality control to ensure tested report in accuracy, quick and fast transmission. The method is also applicable to hepatitis A, C, D, and E.

Description

technical field [0001] The invention relates to a detection technology for five indicators of hepatitis B virus (HBV) markers, which belongs to the working steps of using computer processing enzyme-linked immunosorbent assay (ELISA, enzyme immunoassay) method for colorimetric determination of hepatitis B virus markers . Background technique [0002] As we all know, in addition to rich clinical experience, diagnosis and treatment of diseases are mainly based on test results reports. Therefore, the accuracy and timeliness of test results is an important part of protecting human health and safety. Hepatitis B (commonly known as hepatitis B) is contagious, easy to relapse, and untimely treatment may cause chronic hepatitis B, which will increase the difficulty and cost of treatment, so it is hoped to obtain accurate test results reports as soon as possible during diagnosis, so as to formulate corresponding treatments in time Program. During the trea...

Claims

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Application Information

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IPC IPC(8): G01N33/535G01N33/576
CPCY02A50/30
Inventor 吴淑梅雷长海朱志年沈茜孙巍巍张乐之
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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