Qualitative of beta-sitosterol in sabal fruit extraction and its quantitative detecting method

A technology of saw palmetto fruit and qualitative method, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problems that affect the production and development of small and medium-sized enterprises, the operation has high precision requirements, and is not suitable for small and medium-sized enterprises, and can reach the detection cost. Low, high separation speed, high accuracy effect

Inactive Publication Date: 2005-01-05
方芳
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In the prior art, the qualitative and quantitative detection methods of β-sitosterol in the saw palmetto fruit extract are carried out with capillary gas chromatography (CGC), and the instruments used in this method are relatively expens

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take 300 mg of saw palmetto fruit extract, add 40 ml of 40% methanolic potassium hydroxide solution, reflux in a water bath for 3 hours, take it out and let it cool, transfer the solution to a 125 ml separatory funnel, wash and reflux with 40 ml of water bottle, combined washings; extracted 4 times with ether, 40 ml each time. Wash the combined extracts with water 4 times, 30-40 ml each time, evaporate the extracts to dryness with ether, add ethyl acetate to the residue to dissolve, then transfer to a 5 ml measuring bottle, add ethyl acetate to the mark, as The test solution. Another amount of β-sitosterol reference substance was added with ethyl acetate to make 5 ml of a solution containing 0.16 mg of β-sitosterol reference substance per milliliter as the reference substance solution. Accurately absorb 2 microliters of the test solution, 1 microliter and 2 microliters of the reference substance solution respectively, and cross-spot the samples in a dot-like manner on ...

Embodiment 2

[0025] Take 100 mg of saw palmetto fruit extract, weigh it accurately, add 20 ml of 13% methanolic potassium hydroxide solution, put it in a water bath to reflux for 2 hours; take it out and let it cool. Transfer the solution to a 125 ml separatory funnel, wash the reflux bottle with 30 ml of water, and combine the washings; extract 3 times with ether, 30 ml each time. Combine the extracts, wash 3 times with water, 30 ml each time; evaporate the extracts to dryness with ether, add ethyl acetate to the residue to dissolve, then transfer to a 5 ml measuring bottle, add ethyl acetate to the mark, and use it as the test sample solution. Another appropriate amount of β-sitosterol reference substance was taken, and ethyl acetate was added to make 5 ml of a solution containing 0.1 mg of β-sitosterol reference substance per milliliter as the reference substance solution. Accurately absorb 2 microliters of the test solution, 1 microliter and 2 microliters of the reference substance so...

Embodiment 3

[0028] Basically carry out according to the method described in embodiment 1, but take following parameter and can reach better detection result:

[0029] Saw palmetto fruit extract weighed 200 mg;

[0030] 20 ml of 13% methanolic potassium hydroxide solution;

[0031] The concentration of sulfuric acid-ethanol solution is 10%.

[0032] Heating method: heating at 100°C for 8 minutes.

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PUM

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Abstract

The invention provides a qualitative and quantitative detection method for beta-sitosterol in saw leaf palm fruit extracts. The invention uses thin layer scanning method, the character is: the testing liquid and contrast liquid are produced through weighing, dissolving, refluence, extracting and drying and dissolving process to the saw leaf palm fruit extracts, uses developing agent to develop them on the preprocessed silica gel plate, they are detected under the ultraviolet after being dried and heated. Then carries on scan with fluorescence mode or visible light mode, it can acquire the content.

Description

Technical field [0001] The invention relates to a qualitative and quantitative detection method of a plant extract used in medicine, in particular to a qualitative and quantitative detection method of β-sitosterol in the saw palmetto fruit extract. Background technique [0002] In the prior art, the qualitative and quantitative detection methods of β-sitosterol in the saw palmetto fruit extract are carried out with capillary gas chromatography (CGC), and the instruments used in this method are relatively expensive, and the operation is complicated and cumbersome , The precision of the operation is high, so it is not suitable for small and medium-sized enterprises to adopt, which affects the production and development of small and medium-sized enterprises. Contents of the invention [0003] The purpose of the present invention is to provide a qualitative and quantitative detection method of β-sitosterol in an improved saw palmetto fruit extract, which requires simple equipm...

Claims

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Application Information

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IPC IPC(8): G01N30/90
Inventor 方芳
Owner 方芳
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