Factory production method for Pleurotus tuber- regium (Fr.)Sing and relevant culture medium thereof
A technology of tiger milk mushroom and liquid culture medium, which is applied in the fields of botanical equipment and methods, fungi, horticulture, etc., can solve the problem of sclerotium growth scale, different performance, low yield rate of strain cultivation, and difficulty in large-scale production capacity. and other problems, to achieve the effect of shortening the growth period, accelerating the growth and reproduction speed, and shortening the culture time.
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Embodiment 1
[0022] Embodiment 1, be used for preparing the liquid culture medium of tiger milk mushroom bacterial classification.
[0023] The formula weight ratio components of the liquid medium are: soybean powder 4%; ginseng powder 1%; glucose 5%; cellulose 8%; VB 1 0.5%; Calcium chloride 2%; Magnesium sulfate 2%; Potassium dihydrogen phosphate 2%; Peptone 22%;
Embodiment 2
[0024] Embodiment 2, the preparation method of tiger milk mushroom bacterial classification.
[0025] The main steps include:
[0026] A, preparation liquid culture medium: the liquid culture medium gained in embodiment 1 is used as the culture medium of tiger milk mushroom bacterial classification.
[0027] B, liquid culture medium disinfection: the liquid nutrient base that embodiment 1 gained is packed in the 5000ml conical flask, seals with gauze cotton pad, wraps up kraft paper, boils and sterilizes in sterilizing autoclave (121 ℃) 1 hour, moves into supernatant after cooling Irradiate the UV lamp in the clean workbench for 35 minutes.
[0028] C. Select the sclerotium of the mother species: take the sclerotia of the tiger milk mushroom that is round, firm and fresh, and rinse with water before use.
[0029] D. Disinfection of mother species sclerotia: put the cleaned mother species sclerotia on the ultra-clean workbench, soak in 75% alcohol for 10 minutes with conventi...
Embodiment 3
[0032] Embodiment 3, be used for the solid culture medium of cultivating tiger milk mushroom sclerotia.
[0033] The formula weight ratio components are: 42% of sawdust; 50% of bran; 2% of ginseng leaf powder; 3% of sucrose; and 3% of calcium carbonate. After fully mixing the components in the formula amount, add water to make it moist. Generally, the amount of water added is suitable for holding the culture medium without dripping.
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