Factory production method for Pleurotus tuber- regium (Fr.)Sing and relevant culture medium thereof

A technology of tiger milk mushroom and liquid culture medium, which is applied in the fields of botanical equipment and methods, fungi, horticulture, etc., can solve the problem of sclerotium growth scale, different performance, low yield rate of strain cultivation, and difficulty in large-scale production capacity. and other problems, to achieve the effect of shortening the growth period, accelerating the growth and reproduction speed, and shortening the culture time.

Inactive Publication Date: 2005-01-26
温宝国
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The production method of tiger milk mushroom sclerotium of prior art is to obtain bacterial classification by the tissue separation of sclerotium or sporocarp, and culture with fixed medium, generally exists bacterial classification cultivation time is long, bacterial classification cultivation yield is low, growth period is long , small sclerotium, low yield, etc., and because of the different tissues used, it is easy to cause the growth scale and performance of sclerotia to be different, and long-term use will lead to the degradation of production traits
Therefore, it is difficult for existing technologies to form large-scale production capacity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1, be used for preparing the liquid culture medium of tiger milk mushroom bacterial classification.

[0023] The formula weight ratio components of the liquid medium are: soybean powder 4%; ginseng powder 1%; glucose 5%; cellulose 8%; VB 1 0.5%; Calcium chloride 2%; Magnesium sulfate 2%; Potassium dihydrogen phosphate 2%; Peptone 22%;

Embodiment 2

[0024] Embodiment 2, the preparation method of tiger milk mushroom bacterial classification.

[0025] The main steps include:

[0026] A, preparation liquid culture medium: the liquid culture medium gained in embodiment 1 is used as the culture medium of tiger milk mushroom bacterial classification.

[0027] B, liquid culture medium disinfection: the liquid nutrient base that embodiment 1 gained is packed in the 5000ml conical flask, seals with gauze cotton pad, wraps up kraft paper, boils and sterilizes in sterilizing autoclave (121 ℃) 1 hour, moves into supernatant after cooling Irradiate the UV lamp in the clean workbench for 35 minutes.

[0028] C. Select the sclerotium of the mother species: take the sclerotia of the tiger milk mushroom that is round, firm and fresh, and rinse with water before use.

[0029] D. Disinfection of mother species sclerotia: put the cleaned mother species sclerotia on the ultra-clean workbench, soak in 75% alcohol for 10 minutes with conventi...

Embodiment 3

[0032] Embodiment 3, be used for the solid culture medium of cultivating tiger milk mushroom sclerotia.

[0033] The formula weight ratio components are: 42% of sawdust; 50% of bran; 2% of ginseng leaf powder; 3% of sucrose; and 3% of calcium carbonate. After fully mixing the components in the formula amount, add water to make it moist. Generally, the amount of water added is suitable for holding the culture medium without dripping.

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Abstract

The invention provides a factory production method for Pleurotus tuber- regium (Fr.)Sing and relevant culture medium thereof, wherein the production method mainly comprises the steps of bacterial production and sclerotium cultivation administration, the invention is characterized in that, culturing the fungus core tissue on shaking table in the liquid medium, thus greatly shortening the bacterial culture time. The liquid medium ingredients are also disclosed in the invention.

Description

Technical field: [0001] The invention relates to a factory production method of tiger milk mushroom sclerotia and related culture medium mixture. Background technique: [0002] Tiger milk mushroom sclerotium is a tropical edible fungus, mainly produced in Africa, and also distributed in tropical Asia and Australia. There is a tradition of eating in some countries and regions, and it can also be used as medicine among the people: it is used to treat stomach pain, constipation, fever, cold, edema, chest pain, nervous system diseases, smallpox, asthma, hypertension, diabetes and other diseases, and can promote Fetal development, improve the survival rate of premature infants. With the improvement of people's living standards and the wide application in medicine, the demand for the sclerotium of tiger milk mushroom will be increasing, and the wild tiger milk mushroom sclerotia can not meet people's needs far away. In recent years, there have been reports on the artificial cult...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C12N1/14
Inventor 温宝国赖丽华
Owner 温宝国
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