Liquid submerged culture Chinese caterpillar fungus energy saving method

A technology of liquid submerged culture and Cordyceps sinensis, applied in the direction of fungi, etc., can solve the problems of high power consumption of the agitator, achieve the effects of reducing energy consumption, realizing contact, and solving high energy consumption

Inactive Publication Date: 2005-01-26
CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the fermentation process needs to turn on the stirre

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0014] Example 1

[0015] An energy-saving production method for cultivating Cordyceps sinensis fungus in a 30-ton general-purpose stirred fermenter:

[0016] 1. Cultivate the slanted strains of Cordyceps sinensis into a general-purpose stirring fermenter step by step:

[0017] (1) First-level shake flask seed culture: insert the Cordyceps sinensis slant strain into the shake flask of the first-level seed, the first-level shake flask is a 500 milliliter triangular flask, 100 milliliters of culture medium, and the rotating speed of the rotary shaker is 150 rpm minutes, the culture temperature was 24°C, and the culture time was 60 hours;

[0018] (2) Secondary shake flask seed culture: the first grade shake flask seed is transferred to the second grade shake flask seed with 10% inoculum for cultivation, and its culture condition is identical with the first grade shake flask seed;

[0019] (3) Three-stage shake flask seed cultivation: the second-stage shake flask seeds are conn...

Example Embodiment

[0031] Example 2

[0032] An energy-saving production method for cultivating Cordyceps sinensis fungus in a 30-ton general-purpose stirred fermenter:

[0033] 1. Cultivate the Cordyceps sinensis slant strains step by step into a general-purpose stirring fermenter. (with embodiment 1)

[0034] 2. General-purpose stirring fermenter culture: the total volume of the fermenter is 30 tons, the medium loading capacity is 23 tons, the inoculation amount is 10%, and the CHYLC energy-saving control method is adopted during the fermentation production period: (a) Early stage fermentation: ventilation volume (V / V) 1:0.6; fermentation temperature is 18°C; tank pressure is 0.08Mpa; time is 20 hours; (b) mid-stage fermentation: ventilation (V / V) is 1:1.0; fermentation temperature is 20°C; tank pressure is 0.04Mpa ; The time is 30 hours; (c) late fermentation: the ventilation rate (V / V) is 1:0.4; the fermentation temperature is 20°C; the tank pressure is 0.09Mpa, and the time is 25 hours. ...

Example Embodiment

[0036] Example 3

[0037] An energy-saving production method for cultivating Cordyceps sinensis fungus in a 30-ton general-purpose stirred fermenter:

[0038] 1. Cultivate the Cordyceps sinensis slant bacterial classification step by step to the general stirring fermenter: same as Example 1

[0039] 2. General-purpose stirring fermenter culture: the total volume of the fermenter is 30 tons, the medium loading capacity is 23 tons, the inoculation amount is 10%, and the CHYLC energy-saving control method is adopted during the fermentation production period: (a) Early stage fermentation: ventilation volume (V / V) 1:0.3; fermentation temperature is 28°C; tank pressure is 0.03Mpa; time is 25 hours; (b) mid-term fermentation: ventilation rate (V / V) is 1:0.5; fermentation temperature is 30°C; tank pressure is 0.08Mpa ; The time is 20 hours; (c) late fermentation: the ventilation rate (V / V) is 1:0.9; the fermentation temperature is 32°C; the tank pressure is 0.03Mpa, and the time is 3...

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Abstract

The invention disclose a liquid submerged culture Chinese caterpillar fungus energy saving method comprising the steps of, expanding the Chinese caterpillar fungus inclined surface bacterial to general purpose agitating type fermentation tank, and carrying out (1) earlier stage fermentation, ventilation quantity volumetric ratio 1 : 0.3-0.6, fermentation temperature 18-28 deg. C, tank pressure 0.03-0.08 MPa, fermentation time 15-25 hours, (2) medium stage fermentation, ventilation quantity volumetric ratio 1 : 0.5-1.0, fermentation temperature 20-30 deg. C, tank pressure 0.04-0.08 MPa, fermentation time 20-40 hours, (3) late stage fermentation, ventilation quantity volumetric ratio 1 : 0.4-0.9, fermentation temperature 20-32 deg. C, tank pressure 0.03-0.09 MPa, fermentation time 20-30 hours.

Description

Technical field: [0001] The invention belongs to the field of fungal fermentation industry and relates to an energy-saving method for submerged liquid cultivation of Cordyceps sinensis. Background technique: [0002] Cordyceps sinensis (Berkeley) Sacc.] is a traditional precious Chinese medicine in my country. It is as famous as ginseng and velvet antler, and is listed as the three major tonics. Modern research has found that Cordyceps sinensis not only has obvious effects of improving immunity and anti-cancer, but also is rich in a variety of functional nutrients, making it an ideal nutritious product. At present, the main sources of Cordyceps sinensis are natural collection, solid-state fermentation and liquid submerged fermentation. Among them, the liquid submerged fermentation method is the main way to obtain Cordyceps sinensis on a large scale. [0003] The liquid submerged fermentation method usually utilizes traditional general-purpose stirred fermenters to cultivate...

Claims

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Application Information

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IPC IPC(8): C12N1/14
Inventor 蔡永峰程池黄宇彤岳国海李勣
Owner CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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