Method and apparatus for removing virus and cells from blood by magnetic composite micro-granules

A technology of composite particles and diseased cells, applied in the field of biochemistry, can solve problems that do not belong to the scope of blood virus removal, cannot completely remove viruses, and have not explained the methods of virus and magnetic particle removal.

Active Publication Date: 2005-02-23
XIAN GOLDMAG NANOBIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] A Chinese patent discloses (application number: CN03113522.6) a nanoscale anti-AIDS virus microsphere. The method is to introduce magnetic particles into the vein by injection, and inactivate the virus by inducing heat production, so it cannot completely remove the virus. The virus still exists in the body, and it does not explain the method of removing residual virus and magnetic particles in the body, so this technology does not belong to the scope of blood virus removal
After searching, there is no relevant report on the method of removing viruses or diseased cells in the blood with magnetic particles

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0023] Example 1: This embodiment only illustrates the Fe used 3 o 4 / Preparation of Au-assembled magnetic composite particles, but not limited to the scope described in this example:

[0024] Commercially available magnetic Fe 3 o 4 Nanoparticles, washed repeatedly with high-purity water until neutral, prepared into a suspension; take 25mL of Fe 3 o 4 Suspension, after magnetic separation, discard the supernatant, add H 2 O:C 2 h 5 About 250ml of OH (volume ratio 1:1) mixed solvent, then add 2mL of trimercaptopropyl-3 ethoxysilane, react at 50°C for 10 hours for silanization treatment, after magnetic separation, wash repeatedly with double distilled water until neutral , to complete the surface modification; take 30mL of magnetic particles with functional groups on the surface (solid content is 4mg / mL), magnetically separate to remove the supernatant, then add 100mL of colloidal gold with a particle size of 20nm, and react 5- 8 hours, make it assemble on the surface ...

example 2

[0025] Example 2: This example illustrates the immobilization method of biomolecules, such as antibodies and nucleic acid probes, which have affinity with viruses, virus particles, and virus-infected cells, on the surface of magnetic composite particles.

[0026] Take the immobilization of the specific antibody of HIV virus capsid protein gp120 as an example. It can be immobilized by different methods according to the characteristics of different magnetic composite particles. Mix the antibody and magnetic composite particles in the PBS buffer or Tris-HCl buffer for about 10-30 minutes, and then the antibody can be immobilized on its surface conveniently; The two-step method of the coupling agent immobilizes the antibody through the formation of Schiff base; the magnetic composite particles with carboxyl groups can use water-soluble carbodiimide as the coupling agent to immobilize the antibody on the surface of the particle. Antibody-immobilized magnetic composite particles can...

example 3

[0028] Example 3: This example is used to illustrate the screening of oligonucleotides for specific regions of HIV-1 virus

[0029] The HIV-1 probe sequence can be screened through HIV-1 gene sequence alignment and BLAST software analysis, such as the screening sequence can be used as the complementary strand of the conserved sequence in the 5'LTR region. This sequence exists in different HIV-1 strains, and prior art shows that this sequence has certain application value.

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PUM

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Abstract

A method and equipment for removing virus and pathologic cells from blood by use of the composite magnetic microparticles is disclosed. Said composite magnetic microparticles, whose surface carries the specific moleculae (DNA sequence or antibody), are in sufficient contact with blood for adsorbing the viruses and pathologic cells on their surfaces, and then are magnetically separated from the blood.

Description

technical field [0001] The invention belongs to the improvement of blood purification method and device. Involved in the field of biochemical technology. Background technique [0002] Blood is the basis for the human body to maintain life. The invasion of blood by some pathogenic substances will directly threaten the life and health of the human body. Therefore, it is very important to control the safety of blood products and purify the blood of patients with diseases. For viral infections, most of them can be self-healed within a few weeks, and some viral infections can remove viruses through their own immune system after a few months, such as measles virus, hepatitis B virus, etc. It is difficult to be removed after infection. These viruses mainly include herpes virus, retrovirus, hepatitis C virus, papilloma virus and polyoma virus. These difficult to remove viruses infect blood and further damage blood cells, plasma and plasma Derivatives cause infection (Lutz G Guert...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61M1/00B01J19/00
Inventor 陈超崔亚丽陆祖宏
Owner XIAN GOLDMAG NANOBIOTECH
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