Collodion investment and use in fault anatomy research

Abstract

The invention relates to a method of colloxylin embedding used insurgery and its application in fault anatomy research, which includes the steps that (1) choose specimen anywhere of corpse fixation by formaldehyde; (2) specimen decalcification : use 10% hydrochloric acid to soak specimen fifteen to thirty days; (3) combine colloxylin embedding method with L-type microtome to prepare continuous fault section. Its effect is that compared with common tissue slice, the fault section has continuity and no loss, whose thickness can be chosen willingly during 25mum to 30mum match magnetic resonance image, and it can show clearly any adjoining structure of fault dissection and reflect local fault anatomy relationship of significant clinical meaning. Being five to ten times larger than common fault section observation range and one twentieth to one tenth of thickness, the manufacture of fault section has optimized preservation method of colloxylin section.

Description

technical field [0001] The invention relates to a collodion embedding method in surgery and its application in section anatomy research. Background technique [0002] Collodion, Nitrocellulose or Soluble gun cotton embedding technology is a method for fixing, dehydrating, soaking, embedding, hardening, block making and slicing for single tissue or organ (such as: nerve tissue or heart) specimens. Methods; limited to histology, observation of 3×3mm or 5×5mm field of view under light microscope. The volume of tissue embedding block is generally 2×3×3mm or 3×5×5mm thick, which cannot reflect the clinically significant local cross-sectional anatomical relationship; such as: the distribution relationship between nerves and blood vessels, the distribution of nerves, blood vessels and organs Sectional anatomical relationships. [0003] Sectional anatomy is a science that studies and expresses the normal morphological structure and basic functions of the human body with the method...

AI Technical Summary

Problems solved by technology

For the fault structure with a thickness of 1-2cm, it cannot meet the development needs of imaging and surgery, and cannot provide detailed information, especially the tomographic slice data and difficult surgical approach data that match the magnetic resonance image; the scanning layer of the magnetic resonance image is generally 1.0 -3.0mm

[0018] ② Frozen milling, the milled specimens cannot be kept, only the substrate can be photographed or processed by computer to obtain simulation data

[0019] ③Biological plasticizing wire saw, the thickness of the fault slice is 1.2mm, and the saw path loss is above 0.3mm; it cannot truly reflect the continuity of the slice

The plasticizing technology requires high equipment conditions and is expensive, and it has not been popularized in China at present.