Collodion investment and use in fault anatomy research

A technology of collodion and embedding method, which is applied in the application field of tomographic anatomy research, can solve the problems of high cost, inability to provide, and inability to preserve specimens, etc., and achieve the effect of no sawing consumption

Inactive Publication Date: 2005-03-02
李云生
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  • Summary
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  • Application Information

AI Technical Summary

Problems solved by technology

For the fault structure with a thickness of 1-2cm, it cannot meet the development needs of imaging and surgery, and cannot provide detailed information, especially the tomographic slice data and difficult surgical approach data that match the magnetic resonance image; the scanning layer of the magnetic resonance image is generally 1.0 -3.0mm
[0018] ② Frozen milling, the milled specimens cannot be k

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1, collodion embedding method, embedding method step:

[0034] ① Specimens: any part of the corpse after formaldehyde fixation, such as the head, spine area, pelvis, limb joints, etc.; ② Specimen decalcification: Soak the designed specimens in 10% hydrochloric acid for 15-30 days until bone tissue Acupuncture can be used; ③ The collodion embedding method is combined with the L-shaped microtome made in Germany to prepare serial tomographic slices.

Embodiment 2

[0035] Embodiment 2, collodion embedding method, on the basis of embodiment 1, the volume of the sample is in the range of (50×50×50mm) to (180×180×150mm), and the slice thickness is set at (0.5-1.0mm), or Set at (25-100μm).

Embodiment 3

[0036] Embodiment 3, the application of collodion embedding method in the study of sectional anatomy, on the basis of Example 1 or 2, the steps of preparation and embedding of sectional anatomical tissue blocks

[0037] ① Decalcification of the sectioned anatomical tissue block;

[0038] ②Rinse: After taking out the decalcified tissue block, rinse with circulating water for 24 hours;

[0039] ③ Conventional dehydration;

[0040] ④ Impregnation: first immerse the dehydrated tissue block in 5% collodion, vacuumize it in a vacuum box with a pressure of 66kPa for 30 minutes, let it stand for 1 day, then evacuate it, and then immerse it in 8% collodion after standing for 1 day. Cotton glue; Vacuum twice under the same conditions.

[0041] ⑤ Embedding and block making: 15% collodion is used as the embedding agent, and the formation of the embedding block takes about 15-30 days (this time mainly depends on the evaporation rate of the collodion solvent). The embedding blocks were h...

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PUM

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Abstract

The invention relates to a method of colloxylin embedding used insurgery and its application in fault anatomy research, which includes the steps that (1) choose specimen anywhere of corpse fixation by formaldehyde; (2) specimen decalcification : use 10% hydrochloric acid to soak specimen fifteen to thirty days; (3) combine colloxylin embedding method with L-type microtome to prepare continuous fault section. Its effect is that compared with common tissue slice, the fault section has continuity and no loss, whose thickness can be chosen willingly during 25mum to 30mum match magnetic resonance image, and it can show clearly any adjoining structure of fault dissection and reflect local fault anatomy relationship of significant clinical meaning. Being five to ten times larger than common fault section observation range and one twentieth to one tenth of thickness, the manufacture of fault section has optimized preservation method of colloxylin section.

Description

technical field [0001] The invention relates to a collodion embedding method in surgery and its application in section anatomy research. Background technique [0002] Collodion, Nitrocellulose or Soluble gun cotton embedding technology is a method for fixing, dehydrating, soaking, embedding, hardening, block making and slicing for single tissue or organ (such as: nerve tissue or heart) specimens. Methods; limited to histology, observation of 3×3mm or 5×5mm field of view under light microscope. The volume of tissue embedding block is generally 2×3×3mm or 3×5×5mm thick, which cannot reflect the clinically significant local cross-sectional anatomical relationship; such as: the distribution relationship between nerves and blood vessels, the distribution of nerves, blood vessels and organs Sectional anatomical relationships. [0003] Sectional anatomy is a science that studies and expresses the normal morphological structure and basic functions of the human body with the method...

Claims

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Application Information

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IPC IPC(8): G01N1/06G01N1/28
Inventor 李云生
Owner 李云生
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