Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method and device for layering and fixing amnion and removing cell by using amnion 3D matrix tissue engineering scaffold

A stromal tissue and amniotic membrane technology, applied in tissue culture, biochemical equipment and methods, microorganisms, etc., can solve the problems of not finding better amniotic membranes, the impact of decellularization efficiency, etc., and achieve low cost, simple method, and easy mass production. Effect

Inactive Publication Date: 2005-03-16
GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
View PDF1 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method does not find a better method for how to fix the amnion, resulting in the efficiency of decellularization being affected

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method and device for layering and fixing amnion and removing cell by using amnion 3D matrix tissue engineering scaffold
  • Method and device for layering and fixing amnion and removing cell by using amnion 3D matrix tissue engineering scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] The amniotic membrane 3D matrix tissue engineering scaffold of the present invention is completely decellularized by laying and fixing amniotic membrane through the following steps:

[0015] (1) Take the amniotic membrane and soak the amniotic membrane with 0.2-5% trypsin solution at 37°C for 20-30 minutes;

[0016] (2) Cut the amniotic membrane into a suitable size, then lay it flat and place it on the bracket;

[0017] (3) Start the rotating body under the stent to make the trypsin liquid produce a vortex for washing until the epithelial cells of the amniotic epithelial cell layer and the fibroblasts of the fibroblast layer are all washed away.

[0018] The present invention can also be completed through the following steps:

[0019] (1) Take the amniotic membrane and soak the amniotic membrane with 0.2-5% trypsin solution at 37°C for 20-30 minutes;

[0020] (2) Cut the amniotic membrane into a suitable size, then lay it flat and place it on the bracket;

[0021] (3) Star...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention concerns a method and a device for amnion extension and fixation to de-cell in amnion 3D matrix tissue engineering. Dipping amnion into trypsase solution, extending suspended amnion and clamping and laying on braket, rotating it through rotary body located under bracket. Then, vortex occur in trypsase solution and rinse the amnion, until the amnion epithelium and fibroblast are all washed down. The inventive method is simple, has low cost, and takes advantage of industrialized tissue engineering.

Description

Technical field [0001] The invention relates to the field of tissue engineering for the manufacture of human organs and tissues, in particular to a method and device for laying and fixing amniotic membrane 3D matrix tissue engineering scaffold for amniotic membrane to be completely decellularized. Background technique [0002] Tissue engineering is the science of making human tissues and organs using bioengineering methods, in which scaffolds (extracellular matrix for cell growth) are an important aspect of tissue engineering methods. In addition to chemically synthesized scaffolds and biological extracts, the tissue engineering scaffolds that have emerged so far also have natural hair matrix (such as: using cadaver skin, fascia lata, hair, and amniotic membrane to make scaffolds). For the natural derivation matrix (3D matrix), it contains various integrin biological information required by the cells, so it is the best solution to use the natural derivation matrix to make tissue ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/08
Inventor 雪原
Owner GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com