Method and device for layering and fixing amnion and removing cell by using amnion 3D matrix tissue engineering scaffold

A stromal tissue and amniotic membrane technology, applied in tissue culture, biochemical equipment and methods, microorganisms, etc., can solve the problems of not finding better amniotic membranes, the impact of decellularization efficiency, etc., and achieve low cost, simple method, and easy mass production. Effect

Inactive Publication Date: 2005-03-16
GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
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  • Abstract
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Problems solved by technology

However, this method does not find a better method for how to fix the

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  • Method and device for layering and fixing amnion and removing cell by using amnion 3D matrix tissue engineering scaffold
  • Method and device for layering and fixing amnion and removing cell by using amnion 3D matrix tissue engineering scaffold

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Embodiment Construction

[0014] The amniotic membrane 3D matrix tissue engineering scaffold of the present invention is completely decellularized by laying and fixing amniotic membrane through the following steps:

[0015] (1) Take the amniotic membrane and soak the amniotic membrane with 0.2-5% trypsin solution at 37°C for 20-30 minutes;

[0016] (2) Cut the amniotic membrane into a suitable size, then lay it flat and place it on the bracket;

[0017] (3) Start the rotating body under the stent to make the trypsin liquid produce a vortex for washing until the epithelial cells of the amniotic epithelial cell layer and the fibroblasts of the fibroblast layer are all washed away.

[0018] The present invention can also be completed through the following steps:

[0019] (1) Take the amniotic membrane and soak the amniotic membrane with 0.2-5% trypsin solution at 37°C for 20-30 minutes;

[0020] (2) Cut the amniotic membrane into a suitable size, then lay it flat and place it on the bracket;

[0021] (3) Star...

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Abstract

The invention concerns a method and a device for amnion extension and fixation to de-cell in amnion 3D matrix tissue engineering. Dipping amnion into trypsase solution, extending suspended amnion and clamping and laying on braket, rotating it through rotary body located under bracket. Then, vortex occur in trypsase solution and rinse the amnion, until the amnion epithelium and fibroblast are all washed down. The inventive method is simple, has low cost, and takes advantage of industrialized tissue engineering.

Description

Technical field [0001] The invention relates to the field of tissue engineering for the manufacture of human organs and tissues, in particular to a method and device for laying and fixing amniotic membrane 3D matrix tissue engineering scaffold for amniotic membrane to be completely decellularized. Background technique [0002] Tissue engineering is the science of making human tissues and organs using bioengineering methods, in which scaffolds (extracellular matrix for cell growth) are an important aspect of tissue engineering methods. In addition to chemically synthesized scaffolds and biological extracts, the tissue engineering scaffolds that have emerged so far also have natural hair matrix (such as: using cadaver skin, fascia lata, hair, and amniotic membrane to make scaffolds). For the natural derivation matrix (3D matrix), it contains various integrin biological information required by the cells, so it is the best solution to use the natural derivation matrix to make tissue ...

Claims

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Application Information

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IPC IPC(8): C12N5/08
Inventor 雪原
Owner GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
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