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Hydrogen generating bacteria and its screening method

A screening method, a technique for bacteria, applied in the directions of bacteria, botanical equipment and methods, biochemical equipment and methods, etc.

Inactive Publication Date: 2005-07-27
HARBIN INST OF TECH
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Problems solved by technology

Yokoi once isolated a relatively acid-resistant hydrogen-producing bacterium (Enterobacter aerogenes HO-39), which can grow anaerobically at pH 4.0, but the isolation of acidophilic hydrogen-producing bacteria has not been reported so far

Method used

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  • Hydrogen generating bacteria and its screening method

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specific Embodiment approach 1

[0013] Embodiment 1: The hydrogen-producing bacterium is named as Ethanologenbacterium hit B49 of Harbin Institute of Technology, and the preservation number is CGMCC No.1153.

[0014] A screening method for hydrogen-producing bacteria CGMCC1153, which uses conventional isolation, purification and screening processes.

[0015] 1. Separation, purification and screening: Take 10ml of hydrogen-producing fermented activated sludge from the biohydrogen reactor, put it into a nitrogen-filled Erlenmeyer flask, add glass beads, vibrate on a shaker for 1-2 hours, and then use sterile Doubling dilution with water, then inoculated in solid medium to make a rolling tube, cultured for 7-14 days, picked a single colony with a sterile capillary, and transferred it to the solid medium; repeat the above operation 3-10 times until The colonies in the tube are consistent with the cell morphology under the microscope; transfer the isolated and purified fermented bacteria to liquid medium, shake a...

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Abstract

This invention relates to a hydrogen generating bacteria and its screening method. It is named Ethanologyenbacterium hit B49 with preserving no. CGMCC No.1153. It is cultured in a special medium and has high efficiency of hydrogen generation. The said bacteria is of acid resistance.

Description

Technical field: [0001] The invention relates to a new species of hydrogen-producing bacteria and a screening method for the new species of hydrogen-producing bacteria (Ethanologenbacterium hitB49). Background technique: [0002] As a renewable clean energy production, biological hydrogen production technology has attracted great attention in the world. There are two types of microbial hydrogen production mechanisms: photosynthetic biohydrogen production and fermentation biohydrogen production. Providing sufficient light energy is a necessary condition for photosynthetic microorganisms to produce hydrogen. Due to the limited penetration of light, it takes a lot of energy to provide sufficient light sources. More importantly, the maintenance and management of light sources are very complicated and occupy a lot of space. Larger, making it very difficult to realize industrialized hydrogen production. Hydrogen production by fermentative microorganisms has the advantages of no ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N15/31
Inventor 任南琪林明邢德峰李建政李永峰丁杰王相晶秦智李秋波
Owner HARBIN INST OF TECH
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