Tobacco having reduced nicotine and nitrosamines

A technology of nicotine and tobacco, which is applied in the fields of tobacco, tobacco preparation, and tobacco treatment, and can solve problems such as excessive nicotine

Inactive Publication Date: 2005-08-03
VECTOR TOBACCO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In many cases, nicotine overdose can co-occur with overzealous use of NRT products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Reducing nicotine and nitrosamine levels in tobacco with 2,4-D

[0077] To reduce nicotine and / or nitrosamine levels in cultivated crops or field of tobacco, prepare 2,4-D with wetting agent "Agral 60" (e.g. 0.0006% v / v miscible oil emulsion) Concentrated aqueous solution (such as 10% solution). A control solution containing a wetting agent but no 2,4-D was also prepared. These solutions were diluted to contain 0.1, 1, 10 or 100 ppm of 2,4-D in the final spray solution. Tobaccos of different maturity (some of which have already been picked) are sprayed individually with parallel dilutions of wetting agent (such as Burley, Virginia and Oriental varieties bred in separate plots using conventional techniques for specific varieties) control field or experimental field. Then spray the plants with the above solution until the solution drips. Plants were allowed to continue growing normally for 14 days. Plants were then harvested and sample leaves (from the sam...

Embodiment 2

[0080] Reducing nicotine and nitrosamine levels in tobacco with ethephon

[0081] Control field or experimental field for cultivating tobaccos of different maturity (some of which have been picked) (such as Burley tobacco, Virginia flue and Oriental tobacco varieties cultivated by conventional techniques for special varieties in separate experimental fields). Some unpicked plants were treated with methyl jasmonate (MeJA) alone or with MeJA and ethephon, and the pointed plants were treated with ethephon. Various amounts of MeJA and ethephon were applied, and "Agral 60" prepared in Example 1 was used in some experimental fields. Plants were harvested 1, 2, 3, 4 and 5 days after treatment. The sample leaves were quickly freeze-dried with liquid nitrogen, and the nicotine concentration (pg / mg dry weight) in the sample leaves was determined. Processed leaves (non-green or yellow dry leaves) were obtained by rapid drying processing technology or conventional processing tech...

Embodiment 3

[0083] Reduction of nicotine and nitrosamines in tobacco with benzo[1,2,3]thiadiazo-7-thioxoic acid

[0084] Level

[0085] Control field or experimental field for cultivating tobaccos of different maturity (some of which have been picked) (such as Burley tobacco, Virginia flue and Oriental tobacco varieties cultivated by conventional techniques for special varieties in separate experimental fields). Tobacco fields were treated with the compound Actigard containing benzo[1,2,3]thiadiazo-7-thioxoic acid at different dilutions according to the manufacturer's instructions. Tobacco was harvested 5, 10, 14, and 21 days after tobacco treatment, and sample leaves were snap-dried with liquid nitrogen. The nicotine levels in the sample leaves were analyzed by routine assays. Harvested stems and leaves were routinely processed using the flash drying method or processing techniques for the test varieties to obtain processed leaves (non-green or yellow dry leaves)...

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PUM

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Abstract

Aspects of the present invention concern tobacco having reduced nicotine and nitrosamines, which is used to manufacture consumer tobacco products, and methods to produce such tobacco. More specifically, embodiments include methods to reduce nicotine and nitrosamines in tobacco crops by applying auxin, auxin analogs, and jasmonate antagonists during the cultivation of such tobacco crops. Tobacco generated using said methods and consumer tobacco products obtained therefrom are also embodiments.

Description

Field of invention [0001] The present invention relates to low nicotine and nitrosamine tobacco and methods of producing such tobacco. More specifically, the present invention relates to the reduction of nicotine and nitrosamines in tobacco cultured to produce tobacco products for consumers by applying compounds that modulate gene expression during culture of the tobacco. Background of the invention [0002] Everyone is aware of the health consequences of tobacco consumption, yet many people continue to use tobacco products. The addictive properties of tobacco products are largely attributed to the presence of nicotine. In addition to being one of the most addictive substances known, nicotine is also a precursor to numerous carcinogenic compounds found in tobacco and the human body. [0003] The addictive nature of tobacco products is also partly attributed to the regular use of delivery systems (such as oral fixation associated with smoking or chew...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/06A01N25/00A01G1/00A01N39/04A01N43/82A01N43/828A01N57/18A01N57/20A01N61/00A24B3/12A24B15/28A24B15/30A24D1/00
CPCA24B15/30A24B15/28A01N57/20A24B15/245A01N61/00A01N43/82A24B15/243A01N39/04A01N2300/00A01N43/46
Inventor M·A·康克林Y·李
Owner VECTOR TOBACCO LTD
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