Flow-type imaging particle measurer and its measuring method

A particle and imaging technology, applied in the field of particle measurement, which can solve the problems of increasing the complexity of the instrument and incapable of cell imaging.

Inactive Publication Date: 2005-08-10
TIANJIN UNIV
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Problems solved by technology

Second, from the perspective of leukocyte classification, because the volume of the same cell is different at different stages of its growth, the volume of different types of leukocytes may overlap, for example: primary lymphocytes may have the same volume as late monocytes or Similarly, flow cytometers need to add special dyes to distinguish them, and use fluorescence to identify them, which increases the complexity of the instrument
In addition, laser flow cytometry cannot image cells

Method used

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  • Flow-type imaging particle measurer and its measuring method
  • Flow-type imaging particle measurer and its measuring method
  • Flow-type imaging particle measurer and its measuring method

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Embodiment Construction

[0028] The present invention is described in detail below in conjunction with embodiment.

[0029] Figure 4 gives a picture of different cells at different stages of development, Figure 5 A picture of some of the crystallization in the urine sediment is given. It can be seen from the figure that the types and volumes of cells and urine sediment are quite different, and it is unreliable to identify and classify them only by detecting their volume, but it is not too difficult for trained naked eyes to identify them. The ideal inspection method should be: 1. Observe the cells one by one "with the naked eye"; 2. The number of observed cells should be as large as possible to increase the probability of finding specific cells. If the "robot eye" can be used to replace the naked eye in a short period of time to quickly observe a large number of cells one by one, and intelligently measure and classify the observed cells, the level of clinical inspection will be greatly improved. ...

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Abstract

The present invention discloses a flow type imaging granules measurement equipment. Said equipment includes optical circuit system formed from ligth source, first lens, light diaphragm, semi-transparent semi-reflecting mirror, light barrier, second lens, reflector and their lens; sheathed flow nozzle; and circuit system formed from photosensor, amplifier, trigger signal generator, CCD camera, video interface and computer. Besides, said invention also provides its measurement method, concrete steps and its application.

Description

technical field [0001] The invention relates to particle measurement technology, in particular to a flow imaging particle measurement device and a measurement method. Background technique [0002] Particle measurement technology is used in many fields such as industrial and agricultural production, scientific research and scientific experiments, medical research and clinical medicine, as well as pharmaceutical, chemical, and petroleum processing industries. For example: measuring particles in refined oil during petroleum processing, measuring particles in water during water purification, checking seawater red tide or river water pollution in environmental science, measuring the number, size and white blood cell classification of blood cells in clinical medicine, and urine sediment inspection wait. Due to the limitation of the detection principle followed by the existing technology, most of the existing measuring instruments can only measure indirectly according to the condu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/00G01N15/06G01N21/51
Inventor 朱险峰
Owner TIANJIN UNIV
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