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Tissue culturing method for astragalus root of Radix Astragali

A culture method and technology of root tissue, applied in the field of astragalus adventitious root tissue culture, to achieve the effect of high yield

Inactive Publication Date: 2005-08-17
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Astragalus adventitious root culture has the advantages of fast growth rate and stable content, and has good economic and social benefits, but there is no relevant report on this aspect so far

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0007] Rinse the seeds of Astragalus membranaceus (purchased from the production base of Astragalus membranaceus in Inner Mongolia) with tap water, soak them in 75% ethanol for 20 seconds, then rinse them with sterile water for 2 to 4 times, then soak them in 2% sodium hypochlorite solution for 15 minutes, and wash them with sterile water. After washing with water for 3 to 5 times, inoculate the seeds in hormone-free MS medium, and cultivate for 4 days. After the buds grow to 1.0 to 1.5 cm, cut the middle part of the root into 0.5 cm segments, and transfer to the seedlings containing 2,4 -D 1mg / L and BA 1mg / L MS solid medium, after 20 days, cut the formed callus into small pieces, transfer to MS solid medium supplemented with IBA 2mg / L and BA 0.2mg / L After 20 days of culture, the adventitious roots in the form of fluff are formed, and then the adventitious roots continue to grow, and the adventitious roots that have grown for 20 days are transferred to the liquid medium with th...

example 2

[0009] After washing the seeds of Astragalus membranaceus with tap water, soak them with 72% ethanol for 30 seconds, then rinse them with sterile water for 2 to 4 times, then soak them with 2% sodium hypochlorite solution for 20 minutes, and rinse them with sterile water for 3 to 5 times. Inoculate the seeds in hormone-free MS medium and culture them for 4 days. After the buds grow to 1.0-1.5 cm, cut the root tips into 0.3 cm segments and transfer them to MS supplemented with 1 mg / L NAA and 1 mg / L BA. On solid medium, cut the formed callus into small pieces after 18 days, transfer to MS solid medium supplemented with IBA 2mg / L and BA0.2mg / L for culture, after 18 days of culture, there will be villous Adventitious roots were formed, and then the adventitious roots continued to grow, and the adventitious roots that had grown for 21 days were transferred to the liquid medium with the same composition for culture, and the growth rate was faster.

example 3

[0011] After washing the seeds of Astragalus membranaceus with tap water, soak them in 71% ethanol for 25 seconds, then rinse them with sterile water for 2 to 4 times, then soak them with 2% sodium hypochlorite solution for 17 minutes, and rinse them with sterile water for 3 to 5 times. Inoculate the seeds in hormone-free MS medium and culture them for 4 days. After the buds grow to 1.0-1.5cm, cut the root tips into 0.4cm segments and transfer them to MS solid medium supplemented with NAA 4mg / L. After 17 days, the formed callus was cut into small pieces, and transferred to MS solid medium containing IBA 1mg / L and BA 0.1mg / L for culture. After 19 days of culture, villous adventitious roots were formed, and after that, adventitious roots To continue to grow, the adventitious roots that had grown for 18 days were transferred to the liquid medium with the same composition for culture, and the growth rate was faster.

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PUM

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Abstract

The present invention discloses the process of culturing adventitious root tissue of astragalus, and belongs to the tissue culture of astragalus as one kind of Chinese medicinal materials. The process of the present invention includes soaking astragalus seed in solution with ethanol in 70-75 vol% and sodium hypochlorite in 2 vol% to sterilize and planting in MS culture medium to induce bacteria-free bud; inoculating the cut cotyledon and bud in MS culture medium containing different plant growth regulator to induce callus; transferring the callus in MS culture medium with indolebutyric acid and kinin to induce adventitious root; and finally transferring the adventitious root to liquid culture medium containing the same plant growth regulator for fast growth. The present invention has simple process, high inducing rate, fast adventitious root proliferating speed and other features.

Description

Technical field: [0001] The invention discloses a tissue culture method of astragalus adventitious root, which belongs to the field of traditional Chinese medicine astragalus tissue culture. Background technique: [0002] Astragalus is a perennial herb of the genus Astragalus in the leguminous family, and its main root is used as medicine. Astragalus membranaceus is a top-grade traditional Chinese medicine with a long history of medicinal use and a wide range of clinical applications. Modern pharmacological studies have shown that astragalus has the effects of invigorating qi and strengthening the exterior, diuresis and detoxification, astringing sores and promoting granulation, replenishing qi and invigorating the middle, and can promote the body's humoral and cellular immunity. At present along with health care medicine increasingly popular, the dosage of Astragalus membranaceus is increasing, and the natural resources are very lacking. In addition, Astragalus membranaceu...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 高文远陈海霞郭肖红陈巍
Owner TIANJIN UNIV
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