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Neutralization monoclonal antibody of white spot syndrome virus and preparation method

A monoclonal antibody and virus technology, which is applied in the interdisciplinary field of immunology and virology, can solve the problem of inability to screen, increase the difficulty of preparing shrimp virus-neutralizing monoclonal antibodies, and the research of shrimp virus-neutralizing monoclonal antibodies is blank and other problems, to achieve the effect of uniform size and strong division

Active Publication Date: 2005-08-31
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

So far, no effective subcultured shrimp cell lines have been established at home and abroad, and the neutralizing monoclonal antibodies of shrimp viruses cannot be screened by continuous cell culture, which increases the difficulty of preparing shrimp virus neutralizing monoclonal antibodies and makes The study of shrimp virus neutralizing monoclonal antibody has been blank

Method used

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  • Neutralization monoclonal antibody of white spot syndrome virus and preparation method
  • Neutralization monoclonal antibody of white spot syndrome virus and preparation method
  • Neutralization monoclonal antibody of white spot syndrome virus and preparation method

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Embodiment Construction

[0046] The neutralizing monoclonal antibody against white spot disease virus produced by hybridoma cells; the preservation number of the hybridoma cells is: CCTCC-C200423, and the specific white spot disease virus neutralizing monoclonal antibody F secreted by it (referred to as : neutralizing monoclonal antibody F) has the ability to bind to the adhesion protein on the white spot virus, thereby blocking the adhesion protein of the virus, blocking the adhesion protein of the virus from binding to the virus receptor on the cell membrane of the virus sensitive cell , the function of preventing the virus from invading cells; the well-growing hybridoma cells are uniform in size, round, bright, vigorously divided, and have unlimited division and proliferation capabilities; in the neutralization experiment in crayfish, the neutralizing effect of the neutralizing monoclonal antibody F Obviously, it has the ability to make crayfish with low mortality and long survival time.

[0047] T...

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Abstract

A neutralizing monoclonal antibody F for white spot virus is secreted by the hybrid tumor cell (CCTCC-C200423). Its preparing process includes such steps as purifying the white spot virus, using it as antigen, creating its monoclonal antibody library by immunological method, incubating the monoclonal antibodies in said library by use of the white spot virus, and screening the neutralizing monoclonal antibody by reacting on the cell membrane of spraw's blood cell. It can be used as the raw material of the passive immune vaccine for prawn.

Description

technical field [0001] The present invention relates to the improvement of antibody application technology, in particular to a white spot syndrome virus (WSSV) neutralizing monoclonal antibody secreted by hybridoma cells and its preparation method, which belongs to the intersection of immunology and virology technology field. Background technique [0002] At present, white spot virus disease is one of the serious diseases in prawn farming, which causes large-scale death of prawns and causes huge losses to the prawn farming industry. In recent years, there have been many research reports on white spot virus, but there is no research on the neutralizing monoclonal antibody and its preparation method. So far, no effective subcultured shrimp cell lines have been established at home and abroad, and the neutralizing monoclonal antibodies of shrimp viruses cannot be screened by continuous cell culture, which increases the difficulty of preparing shrimp virus neutralizing monoclona...

Claims

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Application Information

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IPC IPC(8): C07K16/08C07K16/28C12P21/08
Inventor 战文斌姜有声
Owner OCEAN UNIV OF CHINA
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