Neutralization monoclonal antibody of white spot syndrome virus and preparation method

A monoclonal antibody and virus technology, which is applied in the interdisciplinary field of immunology and virology, can solve the problem of inability to screen, increase the difficulty of preparing shrimp virus-neutralizing monoclonal antibodies, and the research of shrimp virus-neutralizing monoclonal antibodies is blank and other problems, to achieve the effect of uniform size and strong division
CN1660909AActive Publication Date: 2005-08-31OCEAN UNIV OF CHINA
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Patent Information

Authority / Receiving Office
CN ยท China
Current Assignee / Owner
OCEAN UNIV OF CHINA
Publication Date
2005-08-31

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Abstract

A neutralizing monoclonal antibody F for white spot virus is secreted by the hybrid tumor cell (CCTCC-C200423). Its preparing process includes such steps as purifying the white spot virus, using it as antigen, creating its monoclonal antibody library by immunological method, incubating the monoclonal antibodies in said library by use of the white spot virus, and screening the neutralizing monoclonal antibody by reacting on the cell membrane of spraw's blood cell. It can be used as the raw material of the passive immune vaccine for prawn.
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Description

technical field

[0001] The present invention relates to the improvement of antibody application technology, in particular to a white spot syndrome virus (WSSV) neutralizing monoclonal antibody secreted by hybridoma cells and its preparation method, which belongs to the intersection of immunology and virology technology field. Background technique

[0002] At present, white spot virus disease is one of the serious diseases in prawn farming, which causes large-scale death of prawns and causes huge losses to the prawn farming industry. In recent years, there have been many research reports on white spot virus, but there is no research on the neutralizing monoclonal antibody and its preparation method. So far, no effective subcultured shrimp cell lines have been established at home and abroad, and the neutralizing monoclonal antibodies of shrimp viruses cannot be screened by continuous cell culture, which increases the difficulty of preparing shrimp virus neutralizing monoclona...

Claims

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