Reagent kit for real-time quantitative detecting epidermal growth factor receptor gene expression level
A technology for real-time quantification of gene expression levels, applied in the field of medical oncology, can solve problems such as patients' reluctance to take risks, tumor rupture and bleeding
Inactive Publication Date: 2010-12-01
董强刚
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A common point of these methods is that tumor tissue of sufficient size (diameter > 5 mm) is required for detection, but advanced tumors are mostly in a high-risk state such as rupture and bleeding, and biopsy to obtain the required tumor tissue may cause serious medical accidents , patients may therefore be unwilling to take the risk
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Abstract
The present invention provides the process of detecting the gene expression level of epidermal growth factor acceptor EGFR gene in cell outside body quantitatively. The process includes the following steps: extracting RNA from the target cell and obtaining cDNA; selecting primer and Taqman probe in the 1744-4871 site nucleotide region of EGFR gene sequence; preparing standard sample based on the nucleotide in 1520-2016 sites of EGFR gene sequence; and performing real-time quantitative PCR analysis on EGFR gene expression by means of Taqman technology. The present invention provides also reagent kit for the real-time quantitative PCR detection of EGFR gene expression level in target cell.
Description
Kit for real-time quantitative detection of epidermal growth factor receptor gene expression level technical field The present invention relates to the field of medical oncology. Specifically, the present invention describes a new method for real-time quantitative PCR detection of epithelial growth factor receptor (EGFR) gene expression. Background technique Malignant biological behaviors such as uncontrolled proliferation, invasion and metastasis of tumor cells are complex events that require the participation of multiple genes, and the synergistic effects of these genes in time and space are regulated by the intracellular signal transmission chain. Therefore, selectively controlling the function of several key signaling molecules and interfering with the normal operation of the signal transmission chain is a development direction of cancer chemotherapy in the 21st century. Epidermal Growth Factor Receptor (EGFR) is an important link in the above signal transmission cha...
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IPC IPC(8): C12Q1/68
Inventor 董强刚
Owner 董强刚