Seedling raising method for non-shell protonema cell of laver

A technology of shell filaments and filaments, which is applied in the field of seaweed seedlings without shell filaments, can solve the problems of large-scale, high efficiency and low cost, and achieve the effects of high seedling efficiency, convenient operation, and broad application prospects

Inactive Publication Date: 2005-11-02
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the following 40 years, the productive seedling cultivation directly from the suspended filaments without shells has become the ideal and goal of Iwasaki himself and phycologists at home and abroad for decades. A large number of research work and scientific researc

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0011] Example 1 Using Porphyra haitanensis 180 strain to raise seedlings

[0012] The culture container is a 40L transparent storage box, which is placed on a 46×133×230cm four-layer structure culture rack according to groups. 8 boxes form a group. One culture rack is placed in the nursery room area of ​​one square meter and is set at a temperature, In the nursery room with adjustable light, nutrition and ventilation, the growth and development of suspended filamentous cells of different types and strains of laver can be regulated by changing the culture conditions. The final density of the suspended filamentous cells in the culture container is 5~ 10g / L (5g in this embodiment, 200g laver filament cells are cultured in each 40L incubator), various regulatory conditions are in effect, among which temperature and light-time conditions are decisive, and nutrition and ventilation conditions play an auxiliary role. Synchronous development can be achieved through the following cultivat...

Example Embodiment

[0015] Example 2 Using Porphyra haitanae 0307 strain to raise seedlings

[0016] The difference from Example 1 is that the amount of filamentous somatic cells suspended in the late culture container is controlled by 10 grams per liter of culture volume; the algal filaments develop at 20°C with 14 hours of long light for 6 weeks, and sporangia branches The development period is 25°C with 12 hours of short light for 3 weeks and 6 days; the ascosporangium development period is 27°C with 8 hours of short light for 2 weeks; the mature period of development is cooled to 24°C with 10 hours of short light for 1 week. After 83 days of incubation, the synchronous growth rate reached 75% by the end of the third stage. The cells were matured after being flushed for 4 days at night in the fourth developmental stage. After flushing to the 7th day, the total amount of released spores was accumulated. Reach 20 million / g cell. On September 19 of that year, 300 million spores per acre were inoculat...

Example Embodiment

[0019] Example 3 Using Porphyra haitanae 0307 strain to raise seedlings

[0020] The difference from Example 2 is: the algal filament development period is 23°C with 18 hours of long light for 26 weeks, the sporangia branch development period is 26°C with 8 hours of short light for 10 weeks; the conchosporangium development period is 27°C. Take 10 hours of short light for 10 weeks; cool to 24°C during mature release and develop with 8 hours of short light for 10 weeks. After 322 days of cultivation, the synchronous development rate reached more than 90% by the end of the third stage, and there was no flushing treatment in the first 8 weeks of the fourth stage. The number of spores released was small and not concentrated. The flushing was increased in days, and a large amount of release began 2 days after flushing, and the total released amount reached 28 million / g cells during the 10-day flushing period. In the same year, 100 million spores were inoculated per mu of net curtain to...

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Abstract

The present invention discloses no-shell filament cell laver seedling growing process. In the laver seedling growing room, and inside the culture container in the optimized temperature, lighting, nutritious and water motion conditions obtained through experiment, the cultured suspension filament cells are controlled to grow to the cell density of 5-10 g/L synchronously. The culture container is box on shelf. In the later stage, the great amount of cell generated conchospores are collected and transferred into sieve net bag, and cells are flushed with flow water at night so as to spread great amount of conchospores in the day. The present invention makes it possible to grow laver seedlings in large scale, high efficiency and low cost. The process is suitable for cultivating laver of different varieties.

Description

technical field [0001] The invention relates to a laver cultivation technology, in particular to a method for cultivating seaweed seedlings without shell filaments. Background technique [0002] Porphyra is the most important artificially cultivated seaweed in the world, and its output value accounts for two-thirds of all cultivated seaweed. So far, more than 99% of the cultivated laver at home and abroad have adopted the traditional shell filamentous seedling technology. The principle is that the fruit spores released by mature laver can penetrate into the shell and grow into shell filaments. After 6 to 10 months When the growth and development reach maturity, the ascospores are emitted and are the seedlings of laver. Cultivating 1 hectare (equivalent to 15 mu) of seaweed requires cultivating 15 square meters of shell filaments in an indoor pool on land, which is called seedling cultivation. There are problems such as time-consuming, laborious, low seedling raising effici...

Claims

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Application Information

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IPC IPC(8): A01G33/02
Inventor 费修绠于义德张京浦彭光邹立红陈兰涛汤晓荣李大鹏王广策秦松
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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