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Seedling raising method for non-shell protonema cell of laver

A technology of shell filaments and filaments, which is applied in the field of seaweed seedlings without shell filaments, can solve the problems of large-scale, high efficiency and low cost, and achieve the effects of high seedling efficiency, convenient operation, and broad application prospects

Inactive Publication Date: 2005-11-02
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the following 40 years, the productive seedling cultivation directly from the suspended filaments without shells has become the ideal and goal of Iwasaki himself and phycologists at home and abroad for decades. A large number of research work and scientific research results have confirmed the cultivation of suspended filaments. The body can also produce normal conchospores, but so far no one has been able to solve the three major practical problems of large-scale, high efficiency and low cost that must be solved for production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Embodiment 1 uses altar laver 180 strains to raise seedlings

[0012] The cultivation container is a 40L transparent finishing box, which is placed in groups on a four-layer structure cultivation rack of 46 × 133 × 230cm, 8 cases are 1 group, and a cultivation rack is placed to occupy one square meter of seedling room area, set at temperature, In the seedling cultivation room with adjustable light, nutrition and ventilation, the growth and development of suspended filamentous cells of different types and strains of Porphyra can be regulated by changing the culture conditions. The final density of suspended filamentous cells in the culture container is 5 ~ 10g / L (the present embodiment is 5g, and each 40L incubator cultivates 200g laver filamentous cells), and each control condition all works, and wherein temperature and light-time condition are decisive, and nutrition and aeration condition play auxiliary role. Synchronous development can be achieved through the followi...

Embodiment 2

[0015] Embodiment 2 uses altar laver 0307 line to raise seedlings

[0016] The difference from Example 1 is that the amount of suspended filamentous cells in the container in the later stage of culture is controlled by 10 grams per liter of culture volume; the algae development stage is 20 ° C with 14 hours of long-term light for 6 weeks, and the sporangia branch During the development period, the temperature was 25°C with 12 hours of short light for 3 weeks and 6 days; during the development of ascospores, the temperature was 27°C with 8 hours of short light for 2 weeks; during the mature and diffuse development period, the temperature was lowered to 24°C with 10 hours of short light for 1 week. After 83 days of cultivation, the synchronous development rate reached more than 75% by the end of the third stage. In the fourth developmental stage, after 4 days of water flushing in the evening, the cells were ripened. After flushing until the 7th day, the total amount of released s...

Embodiment 3

[0019] Embodiment 3 uses altar laver 0307 line seedling cultivation

[0020] The difference from Example 2 is that: the algae filament development period is 23 ° C with 18 hours of long light for 26 weeks; the sporangium branch development period is 26 ° C with 8 hours of short light for 10 weeks; With 10 hours of short light for 10 weeks; during the mature and diffuse development period, the temperature was lowered to 24°C and 8 hours of short light was used for 10 weeks. After 322 days of cultivation, the synchronous development rate reached more than 90% at the end of the third period. In the first 8 weeks of the fourth period, there was no flushing treatment, and the number of spores was less and not concentrated. After flushing was added every day, a large amount of cells began to be released 2 days after flushing, and the total amount of cells released during the 10-day flushing period reached 28 million / g cells. In that year, 100 million spores were inoculated per mu o...

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Abstract

The present invention discloses no-shell filament cell laver seedling growing process. In the laver seedling growing room, and inside the culture container in the optimized temperature, lighting, nutritious and water motion conditions obtained through experiment, the cultured suspension filament cells are controlled to grow to the cell density of 5-10 g / L synchronously. The culture container is box on shelf. In the later stage, the great amount of cell generated conchospores are collected and transferred into sieve net bag, and cells are flushed with flow water at night so as to spread great amount of conchospores in the day. The present invention makes it possible to grow laver seedlings in large scale, high efficiency and low cost. The process is suitable for cultivating laver of different varieties.

Description

technical field [0001] The invention relates to a laver cultivation technology, in particular to a method for cultivating seaweed seedlings without shell filaments. Background technique [0002] Porphyra is the most important artificially cultivated seaweed in the world, and its output value accounts for two-thirds of all cultivated seaweed. So far, more than 99% of the cultivated laver at home and abroad have adopted the traditional shell filamentous seedling technology. The principle is that the fruit spores released by mature laver can penetrate into the shell and grow into shell filaments. After 6 to 10 months When the growth and development reach maturity, the ascospores are emitted and are the seedlings of laver. Cultivating 1 hectare (equivalent to 15 mu) of seaweed requires cultivating 15 square meters of shell filaments in an indoor pool on land, which is called seedling cultivation. There are problems such as time-consuming, laborious, low seedling raising effici...

Claims

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Application Information

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IPC IPC(8): A01G33/02
Inventor 费修绠于义德张京浦彭光邹立红陈兰涛汤晓荣李大鹏王广策秦松
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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