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Quick diagnostic method for differentiating Eperythrozoonosis

A technology for eperythrozoonosis and eperythrozoon, which is applied in the field of veterinary medicine and medical diagnosis, can solve the problems of long detection time and high cost, and achieve the effect of simple operation

Inactive Publication Date: 2005-11-16
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have the problems of long detection time and high cost.
Therefore, there is no fast, simple, accurate and reliable method for detecting Eperythrozoon at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Among 20 dogs with suspected Eperythrozoon disease admitted to the veterinary clinic, blood was collected from the forearm vein of the dogs, and one drop was placed on a glass slide, and diluted with 1:1 normal saline. Under the microscope, it can be seen that Eperythrozoites are mostly ring-shaped, spherical, short-rod-shaped, dumbbell-shaped or star-shaped flash bodies attached to the surface of red blood cells, or free in plasma. There are 1-5 eperythrozoons attached to the surface of each red blood cell in mild infection, 6-8 in moderate infection, and more than 20 in severe infection. After dropping an equal amount of 0.9% hydrochloric acid solution, the Eperythrozoon can be seen to swing, twist, roll, rise and fall under the microscope. After the same amount of 0.1% iodine solution was added dropwise, the Eperythrozoa stopped moving immediately, and after washing with PBS or normal saline, the Eperythrozoa did not recover, nor were they stained by iodine. Finally...

Embodiment 2

[0017] During the epidemic period of canine eperythrozoonosis, aseptic blood was collected from the fingertips of 7 veterinary outpatients and nurses who had long-term contact with the affected dogs, and one drop was placed on the glass slide, and diluted with 1:1.2 normal saline. Under the microscope, it can be seen that Eperythrozoites are mostly ring-shaped, spherical, short-rod-shaped, dumbbell-shaped or star-shaped flash bodies attached to the surface of red blood cells, or free in plasma. There are 1-5 Eperythrozoons attached to the surface of each red blood cell in mildly infected patients, and more than 6 in moderately infected patients. After adding the same amount of 1% hydrochloric acid solution dropwise, it can be seen that the epierythrozoon can swing, twist, roll, rise and fall under the light microscope. After the same amount of 0.1% iodine solution was added dropwise, the Eperythrozoa stopped moving immediately, and after washing with PBS or normal saline, the ...

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PUM

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Abstract

An identification method of erythrocyte inclusion includes placing blood on glass slide, dropping normal saline on it and examining it by microscope for viewing whether there is inclusion or not, dropping hydrochloric or acetic acid on blood with said inclusion and viewing whether there is inclusion to move or not after red blood cell is broken, dropping iodine on blood having inclusion and viewing whether inclusion move is stopped or not, confirming that inclusion is existed if it is neither to restart to move nor to be stained by iodine.

Description

technical field [0001] The invention relates to a method for rapid differential diagnosis of eperythrozoonosis, a zoonotic disease, which can be used for the diagnosis of eperythrozoonosis in animals and humans, and belongs to the technical field of veterinary medicine and medical diagnosis. Background technique [0002] Eperythrozoonosis is caused by obligate blood parasites - Eperythrozoon parasitizing on the surface of human and animal red blood cells, plasma and bone marrow. It is an important zoonotic infectious disease with anemia, jaundice and fever as the basic symptoms. Because the disease has a wide range of distribution, mostly recessive infection, and many infected hosts, it has caused great harm to human health and the development of animal husbandry. [0003] Eperythrozoonosis has become a new global zoonotic disease that has been widely concerned at home and abroad. However, due to the characteristics of Eperythrozoonosis as an opportunistic pathogen, high rec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/49
Inventor 华修国崔立朱建国
Owner SHANGHAI JIAO TONG UNIV
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