Method for preparing nanometer particles of galactose hydroxyapatite-polylysine

A technology for converting hydroxyapatite and polylysine, which can be used in gene therapy, pharmaceutical formulations, powder delivery, etc., and can solve the problems of unsatisfactory results, poor chemotherapy targeting, and low surgical resection rate.

Inactive Publication Date: 2006-02-22
张阳德
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  • Abstract
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AI Technical Summary

Problems solved by technology

At present, conventional clinical treatment has a low rate of surgical resection, poor targeting of chemotherapy, and obvious side effects
In recent years, although gene therapy has made a lot of achievements in basic research, the results of clinical trials are still not satisfactory

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0006] The preparation of a portion of galactosylated hydroxyapatite-polylysine nanoparticles will be described in detail below in conjunction with the examples.

[0007] Synthesis of galactosylated poly-lysine by reductive amination method. Take 200mg of polylysine, 800mg of alpha-lactose, and 500mg of sodium cyanoborate in 200ml of deionized water, and react in an electric heating constant temperature water bath at 37°C for 24 hours. Add appropriate NaOH solution to the reaction solution with a precision acidity meter, and adjust the pH value. Adjusted to 8.5, 37 ° C to continue the reaction for 6h. The reaction solution was dialyzed against water at room temperature to terminate the reaction to remove unreacted lactose, purified through a Sephadex G-25 column, and the purified product was vacuum-dried.

[0008] Take an appropriate amount of hydroxyapatite nanoparticles and wash them five times with deionized water to remove the influence of free ions on the surface zeta po...

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PUM

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Abstract

Disclosed is a process for preparing nanometer particles of galactose hydroxyapatite-polylysine which comprises, rinshing hydroxyapatite nano granules with water, centrifuging and collecting deposition, vacuum drying, grinding, suspending hydroxyapatite nano particle powder deposition into water, carrying out ultrasonic oscillation, charging cerebrose polylysine solution, mixing and stirring, centrifuging to remove the supernatant fluid, suspending the deposition into deionized water.

Description

technical field [0001] The invention relates to a preparation method of nanoparticle of medicine for liver cancer gene therapy. technical background [0002] Primary liver cancer is one of the ten most prevalent malignant tumors in the world. About 386,000 people die of liver cancer every year in the world, of which 45% are in China. At present, conventional clinical treatment has a low rate of surgical resection, poor targeting of chemotherapy, and obvious toxic and side effects. In recent years, although gene therapy has made a lot of achievements in basic research, the results of clinical trials are not satisfactory. Contents of the invention [0003] The object of the present invention is to provide a method for preparing liver cancer gene therapy drug—galactosylated hydroxyapatite-polylysine nanoparticles, which has good chemotherapy targeting and less toxic and side effects. [0004] The technical scheme of the pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/14A61K48/00A61K47/34A61P35/00
Inventor 张阳德
Owner 张阳德
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