Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for making molecular hybridization using stained old cerebotissue section and its use

A technology of molecular hybridization and tissue slicing, which is applied in the field of medical and life science experimental research, can solve the problems that the original tissue specimen cannot be found, has no research value, and cannot obtain new evidence, and achieves low price, clear results and reasonable design Effect

Inactive Publication Date: 2006-05-24
高焕民 +1
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after the H&E stained sections are made, the remaining tissues are generally discarded, so hospitals and other units often keep only such sections, which have no new research value; and the original tissue samples cannot be found, so further new evidence cannot be obtained

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for making molecular hybridization using stained old cerebotissue section and its use
  • Method for making molecular hybridization using stained old cerebotissue section and its use
  • Method for making molecular hybridization using stained old cerebotissue section and its use

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach

[0035] The source of the old slices was taken from the experimental animal macaque (Macaca mulatta), also known as yellow monkey, rhesus monkey, and Guangxi monkey, which belonged to the monkey family; it was divided into the experimental group (8 cases) and the control group (that is, the "sham operation" mentioned in the application document). group", 5 cases), and each group repeated different times according to the experimental requirements. Details are as follows:

[0036] Wash the specimen sections stained with hematoxylin-yinhong (H&E) with 75% ethanol to eliminate the pollutants on the glass slides;

[0037] Remove the gum with xylene to remove the coverslip;

[0038] Formaldehyde is decolorized to remove the dye that has been stained;

[0039] Microwaves are used for antigen retrieval to meet the requirements of new molecular hybridization detection.

[0040] Immunohistochemical staining was performed as follows:

[0041] (1) Sections were fixed in 4% paraformalde...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to experimental investigation area of medicine and life sciences. When original specimen cannot be found, chemical treatment is carried out for dyed slice of dated brain tissue only saved. Thus, new test of molecular hybridization is carried out on dated slice. The method overcomes disadvantages of that slice of brain tissue is one-off utilized traditionally, and it is not possible to be compensated if specimen is lost. The invention is applicable to medical jurisprudence, hospital and research and development section to obtain new evidence from dated slices.

Description

technical field [0001] The invention belongs to the field of medical and life science experimental research, in particular to a method for molecular hybridization detection using old brain tissue slices. [0002] This design overcomes the traditional one-time use and irreparable shortcomings of lost brain tissue slices. The design is reasonable and novel, and it is easy to promote and use in forensic departments, hospitals and scientific research departments, so as to obtain new molecular evidence on old slices. Background technique [0003] Once the brain tissue section has undergone histochemical staining such as hematoxylin-Yinhong staining (commonly known as H&E staining), it will be sealed between the cover slide and the glass slide by resin and cannot be removed for further use. However, after the H&E stained sections are made, the remaining tissues are generally discarded. Therefore, hospitals and other units often keep only such sectio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28C12M1/00C12N1/00C12Q1/68G01N33/53
Inventor 高焕民梁桂霞柳耀泉王少萍孙锋
Owner 高焕民
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com