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Apple raw juice identifying method

A raw juice, apple technology, applied in chemical method analysis, measuring devices, instruments, etc., can solve the problems of lack of systematicness and integrity, prone to misjudgment of results, complex analysis procedures, etc., to achieve simple test methods, eliminate environmental problems The influence of factors, the effect of overcoming limitations

Inactive Publication Date: 2006-05-24
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The currently reported detection methods for the authenticity of apple juice include: sugars, organic acids, phenols, inorganic elements, amino acids and other specific components, and specific instruments such as stable isotopes, spectroscopy, position-specific natural isotope fractionation-NMR methods, etc. Analysis methods and standard sample comparison methods, these methods are not only complicated in analysis procedures, time-consuming and expensive, but also require expensive analytical instruments and equipment, some of which are difficult to complete in ordinary laboratories; at the same time, due to the many components contained in fruit juice , the type and quantity of its ingredients change with the variety, place of origin, climate, farming methods, years, fertilizers, pesticides and other factors, and these existing methods do not consider the influence of these factors, lacking a certain system and Integrity, there are respective limitations in application, and sometimes the results are prone to misjudgment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1, method verification

[0019] 1). Self-made apple juice samples, put them in a refrigerator at 4°C for testing, and use HPLC to analyze and determine the content of D-malic acid in the samples is 0mg / L, that is, no D-malic acid is detected in the samples to be tested. Malic acid, continue to step 2.

[0020] 2).Using the HPLC method to analyze and measure the content of malic acid in the sample is 3.08mg / mL, that is, the content of L-malic acid in the sample is 3.08mg / mL, Y A ≥1.67mg / mL;

[0021] Determination of total acid (X) in the sample by acid-base titration 1 ) is 0.276g / 100mL, X 1 ≥0.15g / 100mL;

[0022] Utilize 3,5 dinitrosalicylic acid colorimetric method to measure the total sugar in the sample, obtain total sugar / total acid (X 2 ) is 36.59, 9.60≤X 2 ≤99.14;

[0023] Determination of fructose (X) in samples by HPLC 3 ) and glucose / fructose (X 4 ), to get fructose (X 3 ) is 5.31g / 100mL, glucose / fructose (X 4 ) is 0.60, 3.34g / 100mL≤X 3 ≤...

Embodiment 2

[0029] Example 2 Identification of the authenticity of "Huiyuan 100% Apple Juice"

[0030] 1) Take 500mL of commercially available "Huiyuan 100% Apple Juice" and place it in a refrigerator at 4°C to be tested. Use HPLC to analyze and determine the content of D-malic acid in the sample to be 0mg / L, that is, it is not detected in the sample to be tested. For D-malic acid, proceed to step 2.

[0031] 2) Utilize the HPLC method to analyze and measure the malic acid content in the sample to be 3.41mg / mL, namely the L-malic acid content in the sample is 3.44mg / mL, Y A ≥1.67mg / mL;

[0032] Determination of total acid (X) in the sample by acid-base titration 1 ) is 0.34g / 100mL, X 1 ≥0.15g / 100mL;

[0033]Utilize 3,5 dinitrosalicylic acid colorimetric method to measure the total sugar in the sample, obtain total sugar / total acid (X 2 ) is 27.66, 9.60≤X 2 ≤99.14;

[0034] Determination of fructose (X) in samples by HPLC 3 ) and glucose / fructose (X 4 ), to get fructose (X 3 ) is...

Embodiment 3

[0040] Example 3 Identification of authenticity of "Great Lakes 100% Apple Juice"

[0041] 1). Take 600mL of commercially available "Dahu 100% Apple Juice" and place it in a refrigerator at 4°C for testing. Use HPLC to analyze and determine the content of D-malic acid in the sample is 0mg / L, that is, it is not detected in the sample to be tested. To D-malic acid, proceed to step 2.

[0042] 2).Using the HPLC method to analyze and measure the content of malic acid in the sample is 5.11mg / mL, that is, the content of L-malic acid in the sample is 5.11mg / mL, Y A ≥1.67mg / mL;

[0043] Determination of total acid (X) in the sample by acid-base titration 1 ) is 0.50g / 100mL, X 1 ≥0.15g / 100mL;

[0044] Utilize 3,5 dinitrosalicylic acid colorimetric method to measure the total sugar in the sample, obtain total sugar / total acid (X 2 ) is 21.00, 9.60≤X 2 ≤99.14;

[0045] Determination of fructose (X) in samples by HPLC 3 ) and glucose / fructose (X 4 ), to get fructose (X 3 ) is 8....

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PUM

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Abstract

The method includes steps: (1) detecting D - malic acid; (2) measuring parameter index and calculating content of malic acid. Mathematic model of character of apple natural juice is a creative application made by the invention. Measuring each parameter index accomplishes measurement for malic acid in sample. The model eliminates influences from each environmental factor, possessing systematicality and all sidedness. Comparing current method, the invention overcomes limitation, inaccuracy and difficult operability and possesses advantages of simple testing method and no need of costly instrument.

Description

technical field [0001] The invention relates to a method for identifying apple juice. Background technique [0002] Juice is the essence of fruit, rich in various nutrients, bright in color and pleasant in flavor, which is favored by consumers. With the improvement of people's living standards and the constant changes in demand, people gradually replace various artificially synthesized drinks with fruit juice and its drinks, and the consumption of fruit juice drinks is increasing. However, due to the limitation of fruit raw materials and production capacity, The production cost of fruit juice is relatively high, which prompts some dishonest manufacturers to add cheap fruit juice to pure fruit juice or use synthetic substances to make fruit juice drinks in order to gain profits. According to statistics, there are 50-80% of the international % of fruit juices are adulterated to varying degrees. In December 2003, the State Administration for Industry and Commerce released the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N35/00G01N21/31G01N30/02G01N31/00
Inventor 胡小松高海燕吴继红廖小军陈芳
Owner CHINA AGRI UNIV