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Imageable animal model of SARS infection

An animal model, animal technology, applied in the field of coronavirus infection models

Inactive Publication Date: 2006-09-06
ANTICANCER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The RNase TI oligonucleotide fingerprint analysis of DIssA showed that gene 1 and gene 7 of DIssA were basically complete, but there were multiple deletions in gene 2 and gene 6

Method used

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Embodiment 1

[0532] A. Background

[0533] Describes a two-color fluorescence imaging model of tumor-host interactions based on RFP-expressing tumors grown in GFP transgenic mice, enabling two-color visualization of tumor-stroma interactions that Interactions include tumor angiogenesis and infiltration of lymphocytes in the tumor. Transgenic mice expressing GFP under the control of the chicken β-actin promoter and cytomegalovirus enhancer were used as hosts (Okabe, M., et al., FEBS Lett (1997) 407:315-319). Under blue excitation light, all tissues in this transgenic line fluoresced green. Mouse melanocyte B16F0 (B16F0-RFP) expressing RFP can be transduced with pLNCX2-DsRed-2-RFP plasmid. B16F0-RFP tumors and GFP-expressing host cells can be clearly visualized simultaneously. High-resolution dual-color imaging can distinguish tumor cells and host tissues down to the single-cell level. Host cells including fibroblasts, tumor infiltrating lymphocytes, dendritic cells, GFP-expressing blood...

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Abstract

The present invention describes an imageable animal model of coronavirus infection.

Description

[0001] related application [0002] This application claims priority under 35 U.S.C § 119(e) to U.S. Provisional Patent Application 60 / 473,691, filed May 27, 2003, which is hereby incorporated by reference in its entirety. technical field [0003] The present invention relates to a coronavirus infection model. More specifically, it concerns animals infected with fluorescent protein-tagged coronaviruses. Background technique [0004] The recent outbreak of Severe Acute Respiratory Syndrome (SARS) around the world has led to massive deaths and disrupted travel plans, and has also put thousands of people in quarantine. In a short time, using clinical samples obtained from patients in six countries, it has been established that the infection is caused by the coronavirus. See, eg, Ksiazek, T.G., et al., New England J. Med. (2003) 348:1947-1958. [0005] Members of the coronavirus family contain a positive-sense RNA genome of approximately 30 kb and can cause respiratory or int...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/10C07K14/00C07K17/00A01K67/00C07H21/04C07K14/165C12NC12Q1/70G01N33/50
CPCA01K2267/0393C07K2319/60A01K2227/105A01K2217/05G01N2333/165C07K14/005G01N33/5082A01K2267/0337C12N2770/20022G01N33/5091
Inventor 杨萌徐明旭
Owner ANTICANCER