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Promoter used for producing industrial enzyme with high prodn. rate

A promoter and hydrolase technology, applied in the field of molecular biology, can solve problems such as multiple copies of the unseen cbh1 promoter

Inactive Publication Date: 2006-09-13
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

But in Trichoderma reesei, there is no report about multiple copies of the cbh1 promoter

Method used

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  • Promoter used for producing industrial enzyme with high prodn. rate
  • Promoter used for producing industrial enzyme with high prodn. rate
  • Promoter used for producing industrial enzyme with high prodn. rate

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0033] The PIcbh1, PIVcbh1, and PVIcbh1 promoters from Trichoderma reesei are all constructed in the present invention, which will be described in detail below.

[0034] (1) Construction of the promoter

[0035] (a) Construction of a promoter that relieves glucose repression

[0036] lmen M et al. (1996) performed a deletion analysis on the cbh1 promoter functional region of Trichoderma reesei and found that the 240bp (-740~-500bp) fragment affected the regulation of the cbh1 promoter on glucose medium and inhibited the expression of the cbh1 gene. Takashima Shou et al. (1996) further analyzed the CreI binding site of the cbh1 promoter and found that the real glucose repression site is the three binding sites between -724 and -684bp upstream of the promoter. Therefore, the present invention constructs a promoter that relieves glucose repression by deleting the 50 bp that contains this region between -724 and -684 bp.

[0037] According to the reported cbh1 promoter sequence of...

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Abstract

The invention discloses a promoter to produce industry enzyme in Trichoderma reesei. The invention recombines dextrose stop point on PCR lack Trichoderma reesei exterior contact dextrose fiber disaccharide hydrolase I gene promoter, and takes alteration to the function area by taking multi copy strategy to gain a series of high promoting activity promoter.

Description

technical field [0001] The present invention belongs to the field of molecular biology. In particular, the present invention relates to a new class of promoters for the production of industrial enzymes for high-efficiency gene expression in Trichoderma reesei. Background technique [0002] With the development of gene recombination technology, people began to use microorganisms to produce various useful proteins and other useful substances. Traditionally, prokaryote Escherichia coli (Escherichia coli) is generally used as a production host, and has been used for the production of various substances at present. However, the protein produced in Escherichia coli sometimes forms insoluble particles in the bacteria, which greatly affects the yield and activity of the protein. Compared with eukaryotes, there is no mechanism for glycosylation of proteins in Escherichia coli, and glycosylation is very important for most proteins (especially proteins with medicinal value), and inco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/31C12N15/24C12N15/63C12N1/15
Inventor 汪天虹刘倜
Owner SHANDONG UNIV
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