Method for screening stripe rust resisting wheat and its special primer

Abstract

The present invention discloses method of screening out stripe rust resisting wheat and its special primer. The primer for screening out stripe rust resisting wheat includes the first primer pair comprising the nucleotide sequences of the sequence No. 1 and the sequence No. 2 and the second primer pair comprising the nucleotide sequences of the sequence No. 3 and the sequence No. 4 in the sequence list. The method of screening out stripe rust resisting wheat includes adopting the genome DNA of the tested wheat as template, performing PCR amplification under the guidance of the first primer pair and screening out amplified product with band of 265 bp size, performing PCR amplification under the guidance of the second primer pair and screening out amplified product with band of 238 bp size as the stripe rust resisting wheat variety. The SSR marks Xcfa2040 and Xbarc32 have the linkage distances of 1.4 cm and 4.8 cm separately. The present invention is significant in screening disease resisting wheat variety.

Description

Technical field [0001] The invention relates to a method for screening wheat resistant to stripe rust and its special primers. Background technique [0002] Wheat stripe rust (Puccinia striiformis f.sp.tritici) is a widespread airborne disease that occurs in almost all major wheat-producing countries, especially in cold and high altitude areas. my country is the world's largest wheat stripe rust epidemic area, mainly in the northwest and southwest regions of China. From the 1950s to the present, my country has had 15 moderate epidemics and 7 large-scale epidemics. Among them, the four pandemics in 1950, 1964, 1990 and 2002 had the largest area and the most serious damage. It reached more than 200 million mu, and the last two cases were more than 110 million mu, and the loss of wheat was 600, 300, 265, and 1 million tons respectively (Wan Anmin et al. Review of the occurrence of wheat stripe rust in my country in 2002. Plant Protection 2003, 29(2): 5 -8). Breeding disease-resistan...

AI Technical Summary

Problems solved by technology

At present, there are 40 named wheat stripe rust resistance genes, which are distributed in 37 loci (with 3 multiple alleles). Most of these named genes are seedling disease resistance genes with physiological specialization. The disease gene is favored by breeders due to its high resistance to stripe rust, but when a variety with a single disease resistance gene is planted in a large area, the directional selection of the pathogen race will be accelerated, and the growth of the dominant race population will be nurtured, resulting in Rust-resistant varieties lose their disease resistance after a few years of large-scale application in production

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