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Method for preparing biodegradable herbicide chlorimuron-ethyl

A chlorpyrifosulfuron fungicide and biodegradation technology, applied in herbicides and algicides, botanical equipment and methods, biocides, etc., can solve problems such as low degradation rate, impact on development, impact, etc. The effect of environmental, crop loss reduction, production cost reduction

Inactive Publication Date: 2006-11-15
NORTHEAST AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

Although the microbial degradation of pesticides has been greatly developed at present, and various microbial strains (including bacteria, fungi, etc.) Affected by lower degradation rate
Biodegradation is the development direction to solve pesticide residues. Although this method is green and environmentally friendly, it affects the development of this technology due to its low degradation rate.

Method used

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  • Method for preparing biodegradable herbicide chlorimuron-ethyl

Examples

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Embodiment Construction

[0021] The following examples describe the present invention in more detail:

[0022] 1. Take the soil that has been applied with chlorimuron-methyl for many years in the field as the treatment object, and dissolve it with sterile saline to obtain a soil suspension;

[0023] The concentration of chlorimsulfuron-methyl added to the peptone medium was 100mg / L. After the medium was sterilized and cooled at 121°C for 20 minutes, the soil suspension was inoculated with an inoculum size of 10%, and the culture medium was shaken at 30°C for 5 day, the culture solution was inoculated in peptone medium with a concentration of 200mg / L of chlorimuron-methyl according to the inoculation amount of 10%, and then cultured in shake flasks at 30°C for 5 days, and inoculated 10 times by analogy, according to the concentration of chlorimuron-methyl The concentration gradient of 100mg / L increases, so that the concentration of chlorimuron-methyl in the final peptone medium reaches 1000mg / L before ...

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Abstract

A process for preparing a biodegradable herbicide includes such steps as taking the soil to which the chlorimuron-ethyl is applied several years, suspending it in aseptic physiological saline to obtain the object to be treated, adding chlorimuron-ethyl to the peptone type culture medium, sterilizing, cooling, inoculating said soil suspension, shake-flask culture, repeating previous 5 steps 10 times, slant culture, class-one amplifying culture, class-two amplifying culture, adding the degradating bacteria, and fermenting culture.

Description

(1) Technical field [0001] The invention relates to a preparation method of a biodegradation agent, in particular to a preparation method of a biodegradation agent capable of degrading the herbicide chlorimuron-methyl. (2) Background technology [0002] Chlorimusulfuron-methyl is a sulfonylurea herbicide developed by DuPont in the United States in the mid-to-late 1980s. Since it was widely used in the 1990s, due to its wide herbicidal spectrum, super high efficiency, high selectivity, strong miscibility, and low price It has low characteristics and has always been the main variety of chemical weeding in soybean fields. In recent years, the application area has expanded year by year, and it disappears in the soil mainly through hydrolysis and microbial degradation. However, because it is not easy to volatilize and photolyze, and has a relatively long residual period, it is easy to cause damage to sensitive crops, which limits its application. At the same time, crop rotation ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N47/36A01P13/00
Inventor 陶波滕春红栾凤侠
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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