Transgenic cow obtaining method

A transgenic and genetic technology, applied in the field of obtaining transgenic cattle, can solve problems such as adverse effects on babies, and achieve the effect of high practical application value and broad market prospects.

Inactive Publication Date: 2006-11-22
李宁
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Problems solved by technology

Long-term repeated use of hormone drugs will have a greater adverse effect on the baby

Method used

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  • Transgenic cow obtaining method
  • Transgenic cow obtaining method
  • Transgenic cow obtaining method

Examples

Experimental program
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Embodiment 1

[0029] Example 1, Production and Molecular Biological Detection of Somatic Cell Cloned β-Lactoglobulin Gene Knockout Transgenic Cattle

[0030] The production process of somatic cell cloned β-lactoglobulin gene knockout transgenic cattle is as follows: figure 1 As shown, the specific process includes the following steps:

[0031] 1. Construction of β-lactoglobulin gene knockout target vector

[0032] The construction process of β-lactoglobulin gene knockout vector is as follows: figure 2 As shown, the specific method is as follows:

[0033] Using the genomic DNA of bovine fetal fibroblasts as a template, primer pLeft upper: 5'-ca gcggccgc tagactgtgtgaatgcccactgac-3' (underlined part of the base is the restriction endonuclease NotI recognition site) and primer pLeft lower: 5'-ctc tcgagc Under the guidance of ttcatggtctgggtgacaatgag-3' (the underlined part is the restriction endonuclease XhoI recognition site), PCR amplified a 2488bp DNA fragment, and the PCR reaction condi...

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Abstract

The invention discloses an obtaining method of gene-transition cow, which comprises the following steps: connecting target carrier with linear beta-milk globulin protein mutation gene with somatic cell through isogenesis recombination; forming beta-milk globulin protein gene silence conduct cell; guiding the conduct cell in the cow enucleated mother cell to rebuild embryo; transplanting the recombined embryo in the pregnant cow uterus. The method makes cow produce more milk without beta-milk globulin protein, which is more nutritious for people.

Description

technical field [0001] The invention relates to a method for obtaining transgenic cattle with improved milk components in the field of animal transgenic technology and cell engineering. Background technique [0002] "A glass of milk strengthens a nation", with the improvement of living standards, people's demand for dairy products is increasing. At present, China has entered a period of rapid growth in demand for dairy products. In recent years, the growth rate of per capita consumption of dairy products has exceeded 10% every year. By 2015, the total consumption of dairy products in China will reach 25 million tons, with an average annual growth rate of 6.8%, higher than the growth rate of 1-2% in the same period in the world. But the production of dairy products in China is still at a relatively backward level. With the increasing demand for dairy products, the quality of dairy products has attracted more and more attention. [0003] It is an indisputable fact that the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/85A01K67/00C12N5/10C12N15/873
Inventor 徐慰倬李宁戴蕴平
Owner 李宁
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