Process for producing scyllo-inositol

Abstract

It is intended to provide a novel NAD<+>-independent myo-inositol 2-dehydrogenase which converts myo-inositol into scyllo-inosose in the absence of NAD<+>; a novel enzyme scyllo-inositol dehydrogenase which stereospecifically reduces scyllo-inosose into scyllo-inositol in the presence of NADH or NADPH; and a novel microorganism which belongs to the genus Acetobacter or Burkholderia and can convert myo-inositol into scyllo-inositol. By using these enzymes or the microorganism, scyllo-inositol is produced. Further, scyllo-inositol is purified by adding boric acid and a metal salt to a liquid mixture containing scyllo-inositol and a neutral saccharide other than scyllo-inositol to form a scyllo-inositol / boric acid complex, separating the complex from the liquid mixture, dissolving the thus separated complex in an acid to give an acidic solution or an acidic suspension and then purifying scyllo-inositol from the acidic solution or the acidic suspension.

Description

technical field

[0001] The present invention relates to a process for the production of scyllo-inositol from inositol by microbial conversion.

[0002] The invention also relates to novel NAD + Independent inositol 2-dehydrogenase and its preparation method. The present invention also relates to NAD + A screening method for microorganisms used to prepare blue crab inositol using the activity of independent inositol 2-dehydrogenase as an index. The invention further relates to the use of NAD + The preparation method of scyllo-inositol and scyllo-inositol of independent type inositol 2-dehydrogenase or the highly active strain of the enzyme.

[0003] The invention also relates to a novel enzyme, i.e., scyllo-inositol dehydrogenase, and a method for preparing scyllo-inositol using the enzyme. In more detail, it relates to: a novel enzyme scyllo-inositol dehydrogenase that catalyzes the oxidation-reduction reaction between scyllo-inositol and scyllo-inositol, and stereospecif...

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